Detailed information of TA system TA189327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3082521..3083208
• Replicon: chromosome
• Accession: NZ_CP069069
• Organism: Mycobacterium tuberculosis strain N1176

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JN144_RS14655
• Protein ID: WP_003414064.1
• Coordinates: 3082813..3083208 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN144_RS14650
• Protein ID: WP_003414061.1
• Coordinates: 3082521..3082787 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN144_RS14620	3078160..3079062	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN144_RS14625	3079131..3079883	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN144_RS14635	3080127..3080402	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN144_RS14640	3080399..3082021	-	1623	WP_003909739.1	class I SAM-dependent DNA methyltransferase	-
JN144_RS14645	3082108..3082524	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
JN144_RS14650	3082521..3082787	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN144_RS14655	3082813..3083208	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN144_RS14660	3083205..3083474	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN144_RS14665	3083484..3084578	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN144_RS14670	3084575..3084994	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN144_RS14675	3084993..3085067	+	75	Protein_2896	hypothetical protein	-
JN144_RS14680	3085068..3085547	-	480	WP_003414073.1	dihydrofolate reductase	-
JN144_RS14685	3085618..3086418	-	801	WP_003911953.1	thymidylate synthase	-
JN144_RS14690	3086574..3087311	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T189327 WP_003414064.1 NZ_CP069069:c3083208-3082813 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T189327 NZ_CP090732:c2308127-2307720 [Burkholderia cepacia]
ATGAAGTTCCTGCTGGATACCAACGGGGTGATCGCCATTCTCAAGGGCGAGCCGGCCATCCTGTCCCGACTGCGGTCGCA
TCAGCCGGGCGGTTTCGGGATACCGGCGATCGTCGCGCATGAACTCTATTACGGCGCCTGCAAGAGCCAGCGAGTGACGG
CGAACGTGGCGCGTGTCGAGGCGCTTCAGTTCGAGGTCGTATCGTTCGATGTCGAGGATGCGCAGCATGCCGGCGAAATT
CGCGCACATCTGATCGCGGCCGGTACGCCGATCGGCCCTTACGACGCATTGATCGCAGGGCAGGCACGCGCGCGGCAACT
GGTGCTGATCACGCATGACATCCGGGAATTCGAGCGCGTCCCGGGGCTGCAGGTCGAGGATTGGCTCGCTGAGCCACGCG
AAAACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189327 WP_003414061.1 NZ_CP069069:c3082787-3082521 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189327 NZ_CP090732:c2308366-2308124 [Burkholderia cepacia]
ATGGACATTGCCAAGATCTTCAAGCATGGCGGGTCGCAGGCCGTCCGGCTGCCGAAGGATTTCCGTTTCGACACGGCCGA
GGTCAGGATTCGCCGCCACGGATCGTCGGTCGTTCTCGAGCCGGTGCCGCAGGACTGGGCATGGCTGACGCCGCTGATCG
GGCCGGTCGACGCCGACTTCGAGGCTGCCGCGACCACGCAGCCCTCCGCGCAGGAGCGGCCGGATCCGGACGTATTCGAA
TGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64