Detailed information of TA system TA189324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2942733..2943447
• Replicon: chromosome
• Accession: NZ_CP069069
• Organism: Mycobacterium tuberculosis strain N1176

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: JN144_RS13820
• Protein ID: WP_003413460.1
• Coordinates: 2943007..2943447 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: JN144_RS13815
• Protein ID: WP_003413456.1
• Coordinates: 2942733..2943020 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN144_RS13780	2938155..2938400	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN144_RS13785	2938397..2938801	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
JN144_RS13790	2939018..2939638	+	621	WP_003909700.1	DUF4178 domain-containing protein	-
JN144_RS13795	2939649..2940143	+	495	WP_003909701.1	DUF2617 family protein	-
JN144_RS13800	2940140..2940571	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
JN144_RS13805	2940596..2941054	+	459	WP_070892310.1	DUF350 domain-containing protein	-
JN144_RS13810	2941051..2942622	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
JN144_RS13815	2942733..2943020	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
JN144_RS13820	2943007..2943447	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN144_RS13825	2943468..2944223	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
JN144_RS13830	2944356..2944952	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
JN144_RS13835	2944960..2945805	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
JN144_RS13840	2945834..2946733	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
JN144_RS13845	2946861..2947535	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T189324 WP_003413460.1 NZ_CP069069:2943007-2943447 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T189324 NZ_CP090732:1587886-1588302 [Burkholderia cepacia]
GTGTATTTGATTGATACCAACGTCATCAGTGAAATCAGGAAGGGCAAGCGAACCAACCGTGGCGTGCGGGCGTTCTTCAG
GCAGGCCGCGGCGGACGCCAGCCCGCTTTACCTGTCCGTCGTGACAGTTGCTGAACTTCGCCGCGGCGTCGACCTGATCC
GTCATCGCGGCGATCACCCCCAGGCGTCGGCGCTCGAGGCCTGGGTGGCAATGATCCTGGCCGGCTATGCACCGAACATC
CTGCCGGTCGACATCGAGATCAGCCAGACGTGGGGGCATTTGCGCGTGCCCGATCCGACGCACGAACTCGACAAGCTGAT
CGCGGCCACCGCGCTGATCAACGATCTCACCGTGGTTACGCGCAACGTCGCGGATTTCGCCCGCACCGGCGTCAGGTTGC
TGAACCCGTTCGACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT189324 WP_003413456.1 NZ_CP069069:2942733-2943020 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT189324 NZ_CP090732:1587663-1587899 [Burkholderia cepacia]
ATGGCAAATCTACTGGTACGTAACGTGGATGACAATATCGTTCAGAGCCTGCGTGAGCGGGCTGCGGCAAATGGCCGGAG
TGCCGAGGCCGAACATCGGGCCATTCTGGCCGATGCGCTCGGCAGGCCGAAGCGAAAGACATTTGCGCAAGTGCTGATGA
GCATGCCCGATGTCGGCGAAGACGCGGATTTTCAACGTATTCAGGATTCCGGCGAGGCCAGGCGTGTATTTGATTGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8