Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2882433..2883070 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN144_RS13555 Protein ID WP_003413180.1
Coordinates 2882433..2882828 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN144_RS13560 Protein ID WP_003413183.1
Coordinates 2882825..2883070 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS13515 2878518..2879177 + 660 WP_003900846.1 LppA family lipoprotein -
JN144_RS13520 2879174..2879833 + 660 WP_003900847.1 LppA family lipoprotein -
JN144_RS13525 2879830..2880492 + 663 WP_003900848.1 LppA family lipoprotein -
JN144_RS13530 2880489..2880767 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN144_RS13535 2880860..2881273 + 414 WP_003413164.1 PIN domain nuclease -
JN144_RS13540 2881312..2881569 + 258 WP_003909690.1 CopG family transcriptional regulator -
JN144_RS13545 2881566..2881943 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN144_RS13550 2881959..2882333 - 375 WP_003413177.1 hypothetical protein -
JN144_RS13555 2882433..2882828 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN144_RS13560 2882825..2883070 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN144_RS13565 2883481..2883900 - 420 WP_003413190.1 A24 family peptidase -
JN144_RS13570 2883912..2884721 - 810 WP_003413193.1 shikimate dehydrogenase -
JN144_RS13575 2884718..2885971 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN144_RS13580 2885964..2886476 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189323 WP_003413180.1 NZ_CP069069:c2882828-2882433 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189323 NZ_CP090732:1224909-1225805 [Burkholderia cepacia]
ATGAGTGTCATCGACAAACGCGAGCGCGACCGCACCGCGGCGTTCGCCGCGCTGCTCGGGCAGTTTCCCGAGCAGCGTTG
CGCGGCCGGCGCGGCGGGCACGATCGGTTACCGTGAGGCCGGCGCGCAGCATGCGGGCCGCGCGCTGCCCGTCGTGCTGC
TGCACGGCATCGGCTCGGGCGCCGCATCGTGGGTCAGCCAGCTCGACACGCTCGGCGCATCGCGGCGCGTGCTCGCGTGG
GACGCGCCCGGTTACGGCGTGTCGACGCCCGTGCACGGCGCGTCGCCGGCCGCCGCCGATTACGCGGCATCGCTGAACGC
GTGGCTCGAGGCACTCGGCATCGAACGCTGTGTGCTGGTCGGCCATTCGCTCGGCGCGATCGTCGCGGGCGGTCTCGCCC
GCGTGATGCCCGCGCGGATCGCCGGCCTGCTGCTGGTGTCGCCCGCGGGCGGCTACGGCAGCGCACCGGCCGAAACGCGC
GAATCGCGCCGCGACGCGCGGCTCGCGATGCTCGCCGAACTCGGCCCGGCCGGGCTCGCCGAACAGCGCAGCGGCAACAT
GCTGTCCGGTTTCGCGAACGAGGAGGCACGTGCGTGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTTGCGAACGCCGATCTCGCGGCCGATCTCGCCGGCTTTCGCGGCCGCACGGCCGTGGCCGTCGGC
GCGAACGACGCGATCACGCCGCCCGCCGCGTGCGAGCGGATCGCCGCGGCCGCGCACGTCGGGCTGCAGGTGATTCCGCA
AGCCGGTCATGCCGGTTATGTCGAGGCGCCGGCCGTCTATTCCGCGCTGATCGACTCGTTCTGCCGTCATTGCGACCAAC
AGCGGGGGCTGGCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189323 WP_003413183.1 NZ_CP069069:c2883070-2882825 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189323 NZ_CP090732:1225802-1226659 [Burkholderia cepacia]
ATGAACGAACCGCTGCAACGACCCGAACCCGAAAACGACAAGGCCGAAGCCAGCTACGTGGTGCCTGGCCTCGAACGCGG
GCTGCGGATCCTCGCCGAATTCTCGCCGCGCGAGCCCGTGCTCGGTGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGC
GCACGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTCCTCGAGCGCGCGGACAAGGATCGCAACTACAAG
CTCGGCATCGCCGTGCTGCGGCTCGGCTTCGAATACCTGAGCTCGCTGGAGCTGACCGATCTCGGCCTGCCCGTGATCGA
GAGCCTGCGCGACGCCACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGAGACGTGGTGTTCGTCGCGAAGGCAC
AGAGCCAGTCGCCGGTGTTCAGCTCGATCCGCGTGAACGTCGGCACGCGCTTGCCCGCGCATGCGACGACGCACGGCCAC
GTGCTGATGGGCGACCTGTCACTGAAGGAGCTGCGCTCGTTGTATCCGGAAGGCACGCTGAACCGGATGACGAACGCGAC
ACCGGAAACGGTCGACGCGTTGTACGAAGTGATCCGCGAGGATGCGCTGCGCGGCTACGGCGTCAGCAATTCGTCGTTCG
AGCGCGGCATCTCGGTGGTCACGGCGCCGGTGCGCAACGAGACGCAGAAGATCGTCGCGTGCATCACGGTGACGGTGCCG
CGTCCGGAAATCGACGCGGCGCTGATCGCGGACGGGCTGATCGACAAGGTGCAGCGCGCGGCGGCGGAACTGTCGCGGCG
GCTCAATTACCGCTCGGATGACGAGCACACGTTTATGAAAGCATTAGGACTCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References