Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2336795..2337431 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag JN144_RS10955 Protein ID WP_003410654.1
Coordinates 2337021..2337431 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag JN144_RS10950 Protein ID WP_003410651.1
Coordinates 2336795..2337028 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS10935 2332243..2332644 + 402 WP_009937711.1 metal ABC transporter permease -
JN144_RS10940 2332645..2333049 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
JN144_RS10945 2333133..2336717 - 3585 WP_003909567.1 cobaltochelatase subunit CobN -
JN144_RS10950 2336795..2337028 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
JN144_RS10955 2337021..2337431 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
JN144_RS10960 2337415..2338506 + 1092 WP_003900454.1 precorrin-3B synthase -
JN144_RS10965 2338516..2339142 + 627 WP_003410658.1 precorrin-8X methylmutase -
JN144_RS10970 2339139..2340665 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
JN144_RS10975 2340611..2341834 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T189316 WP_003410654.1 NZ_CP069069:2337021-2337431 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T189316 NZ_CP090730:83650-84051 [Burkholderia cepacia]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATTTGATGAAGCATCAGCCGGAACAGGTCGCGAAACGATTTGC
CCAGTGCTACACGGGGGACGTCGTGATGTCGGCGATTACGTACGCCGAGCTCGAATACGGCGTGGCCGCGTGTGCGAATG
CCGTCCGGGAGCGACGCAACCTTGCCGACCTGATCGAGGATATTCCGGTTGCGCCGTTCGACGCGGCGGCCGCGCAAGCG
TACGGCCCTGTTCGCGAAGCGACCCGCGAGCGGAAGAGGGATCATCTCGACAAACTGATCGCGGCGCACGCGGTGTCGCT
CGATGTCGTGCTCGTGACCAACAACGAGCGGGATTTCGCGAGTTACCCGGGGTTGCGGCTGGAGAACTGGCTGAACGACT
AG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT189316 WP_003410651.1 NZ_CP069069:2336795-2337028 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT189316 NZ_CP090730:83414-83650 [Burkholderia cepacia]
ATGCATACCACGAGGGTGTTCCGGAACGGCAACTCGCAGGCCGTACGGATTCCGGCGGATCTTGCCTACGAGCGCAGCGA
TATCGAGCTCGAAATCGAACGGATCGGCGACGAAATCCGTATTCGTCCCGTGCGACGGCCGTTGGCTGGCGTGCTCGAGA
AGTTCGCGAAATTCGCACCGGATTTCATGGCTGAAGGGCGTGGCGACCAGGAGCAGGCCGACCGCGAGGGTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References