Detailed information of TA system TA189314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2269011..2269637
• Replicon: chromosome
• Accession: NZ_CP069069
• Organism: Mycobacterium tuberculosis strain N1176

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN144_RS10665
• Protein ID: WP_003410075.1
• Coordinates: 2269239..2269637 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN144_RS10660
• Protein ID: WP_003911750.1
• Coordinates: 2269011..2269238 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN144_RS10650	2267050..2267394	-	345	WP_003410065.1	ferredoxin family protein	-
JN144_RS10655	2267583..2268752	-	1170	WP_003899126.1	ATP-binding protein	-
JN144_RS10660	2269011..2269238	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN144_RS10665	2269239..2269637	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JN144_RS10670	2269820..2270251	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN144_RS10675	2270352..2270786	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN144_RS10680	2271223..2271402	-	180	Protein_2110	hypothetical protein	-
JN144_RS10685	2271490..2272110	+	621	WP_031667492.1	transposase	-
JN144_RS10690	2272064..2272654	+	591	WP_003899131.1	IS110 family transposase	-
JN144_RS10695	2272782..2274038	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T189314 WP_003410075.1 NZ_CP069069:2269239-2269637 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T189314 NZ_CP090728:c2354689-2354276 [Burkholderia multivorans]
ATGAGCGGGGCTGACAAGGCGAAAGTGTTTGTGGACAGCAATGTCGTGCTGTATCTGCTGTCCGGCGACGCCGCGAAGGC
GGATCGTGCGGAGGCTCTGTTGCGGCGCCGGCCGACGATCAGCGTTCAGGTGCTGAACGAAGTCACGAATGTCTGCGTGC
GTAAGCTCGGGATGGCGTGGCGTGAGGTCGATCAGTTCCTGGAACTGGTGCGGCAGTTTTGCGTGGTCGCGCCGCTGACA
ATCGAGATGCATGATTTGGCGCGGCGCATCGCCGAGCGCTATGGCCTGGCGTTCTATGATGCGTGCATCGTAGCAGCCGC
GTCGATCGAAGGATGCCACGCCCTCTACACGGAAGACATGCATCACGGATTGATCATCGAAGAGAGCTTGACGTTGCGTA
ACCCTTTCGCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT189314 WP_003911750.1 NZ_CP069069:2269011-2269238 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT189314 NZ_CP090728:c2354909-2354676 [Burkholderia multivorans]
ATGCAGGTTGCTAAATGGGGCAATAGCCTTGCCGTCCGCCTTCCGTCGAGCCTGGTCGAAGCGCTCGAGCTGCGTGAGGG
GGACGATATCGAGATCGTCATAGACGCGCCGCGCACCTTTGCGGTACGCAAGAAACCGGGGGCGGATGAGCTGCTGGAGC
GGTTGCGCCACTTCCGCGGCAAGCTGCCGGCCGATTTCAAGTTCAGCCGGGACGAGGCGAATGAGCGGGGCTGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8