Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2248584..2249170 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag JN144_RS10575 Protein ID WP_003410010.1
Coordinates 2248584..2248928 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag JN144_RS10580 Protein ID WP_003410014.1
Coordinates 2248922..2249170 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS10540 2244289..2244888 + 600 WP_003409989.1 L-lysine exporter -
JN144_RS10545 2245304..2245732 + 429 WP_003409992.1 cellulose-binding protein -
JN144_RS10550 2245958..2246497 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
JN144_RS10555 2247017..2247577 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
JN144_RS10560 2247574..2247915 - 342 WP_003410003.1 DUF2384 domain-containing protein -
JN144_RS10565 2248001..2248258 + 258 WP_003410006.1 hypothetical protein -
JN144_RS10570 2248159..2248494 - 336 WP_003410009.1 dehydrogenase -
JN144_RS10575 2248584..2248928 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
JN144_RS10580 2248922..2249170 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
JN144_RS10585 2249270..2250082 - 813 Protein_2091 cation transporter ATPase CptG -
JN144_RS10590 2250033..2250923 + 891 WP_078441325.1 universal stress protein -
JN144_RS10595 2251125..2253842 + 2718 WP_003410027.1 cation-transporting P-type ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T189313 WP_003410010.1 NZ_CP069069:c2248928-2248584 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T189313 NZ_CP090728:c1023697-1023320 [Burkholderia multivorans]
ATGGGTATAAATTCATCGATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGCTTCGGATCGGCCGGCGTGCTCGAGGTCGTGGAGTCGGAGGACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGCGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT189313 WP_003410014.1 NZ_CP069069:c2249170-2248922 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT189313 NZ_CP090728:c1023320-1022997 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAAATCATCAAGACCCGCAAATGGACGCAGCAGCACGCGGCACGATTGCTCGGCATGA
CGCAACCGAAGTTATCGAACATGCTGCGCGGGCAGTTTCGCGGTGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References