Detailed information of TA system TA189308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2210370..2211073
• Replicon: chromosome
• Accession: NZ_CP069069
• Organism: Mycobacterium tuberculosis strain N1176

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN144_RS10300
• Protein ID: WP_003409778.1
• Coordinates: 2210370..2210699 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN144_RS10305
• Protein ID: WP_003409780.1
• Coordinates: 2210696..2211073 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN144_RS10280	2206753..2207823	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN144_RS10285	2207820..2208335	+	516	WP_003409718.1	flavin reductase family protein	-
JN144_RS10290	2208332..2209393	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN144_RS10295	2209390..2210160	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN144_RS10300	2210370..2210699	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN144_RS10305	2210696..2211073	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN144_RS10310	2211070..2211660	-	591	WP_003909548.1	SEC-C metal-binding domain-containing protein	-
JN144_RS10315	2211715..2213079	+	1365	WP_003909549.1	HNH endonuclease signature motif containing protein	-
JN144_RS10320	2213234..2213686	-	453	WP_003899095.1	lipoprotein	-
JN144_RS10325	2213750..2214151	+	402	WP_003409869.1	hypothetical protein	-
JN144_RS10330	2214144..2214326	-	183	WP_003409870.1	hypothetical protein	-
JN144_RS10335	2214440..2214790	-	351	WP_003409871.1	hypothetical protein	-
JN144_RS10340	2214801..2215703	-	903	WP_003409874.1	hypothetical protein	-
JN144_RS10345	2215724..2215915	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189308 WP_003409778.1 NZ_CP069069:c2210699-2210370 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189308 NZ_CP090727:1017580-1018155 [Burkholderia multivorans]
ATGACGCATTCGCCATTTACGGCGATCTACGATGCCTGTGTGCTTTATCCCGCGCCGCTTCGCGATTTCCTGATGCGGCT
GGCGTTGAGTGGATGTTTCCGGGCGCGGTGGAGCGCACATATTACCGACGAGTGGAAGCGCAATCTGTTGGACAACCGTC
CGGATTTGACAGCGGAACAGCTTGATCGAACCTCAAGTTTGATGGATCGAGCGATACCGGATGCCGTCGTGACCGGTTAT
GAATCGTTGATTGCCGGGCTTGTTCTCCCGGATGCCGACGATCGTCACGTGCTTACCGCTGCAATCCGTTGCAACGCCAG
CGTGATCGTGACGTTCAATCGAAAGGACTTTCCGGAAGAGGCGTTGTTGCCGTACGGCATCGAGGCCCAGCATCCAGACG
AGTTCGTCGATGATCTTTTCGACCTCGAACCCGCGGTGGTCGTTGCGGCCGCGCGACGACAGCGAGAGGCGCTTAAACAA
CCACCGTTGACTGCAGACCGGTACCTCGAGATCTTGCGACGACAAGGCATGGTGCAGACGTGTCAGTCGCTCGACGCCTA
TCGAGCACTTCTTTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189308 WP_003409780.1 NZ_CP069069:c2211073-2210696 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189308 NZ_CP090727:1017116-1017583 [Burkholderia multivorans]
ATGCATCACGCCACGACACCCGACATGATCTTGCCCGTCGAGCGCGAGATGCAGGCGGCCGTCGAGGCGCAGCGCATGCT
GGCGGCTTATCTCGCAACACGGACCGATACGCAGCGCATTCAGATTTTTGATGACGAGAACCGAGCCCATCAGGTCGAGC
TGCCGACGTCCGCACTTCGTCTGCTGGTGGATATTCTCGCGGAATTGGCGGATGGCAACGCTGTCAAGGTTGTGCCGGTT
CATGCGGAATTGACGACGCAGGAAGCGGCAGATCTGCTCAATGTCTCCCGCCCGCATCTGATCAAGCTGCTTGAGTCGAA
TGCATTGCCCTATCACCGGACGGGCAAGCATCGGCGCGTGCGGTTTTCGGATCTGATGCGCTACAAGGCTGAGCGCGACC
AAGAGAGTGCTGACGCAATGGAAGAACTGAGCAAGCAGGCACAGGATCTGCGGATGGGCTATGAATGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8