Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1692487..1693103 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JN144_RS07975 Protein ID WP_003407593.1
Coordinates 1692786..1693103 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag JN144_RS07970 Protein ID WP_003900349.1
Coordinates 1692487..1692789 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS07960 1688373..1690220 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JN144_RS07965 1690221..1692473 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JN144_RS07970 1692487..1692789 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JN144_RS07975 1692786..1693103 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JN144_RS07980 1693100..1694104 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JN144_RS07985 1694157..1695446 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
JN144_RS07990 1695519..1696136 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
JN144_RS07995 1696350..1696562 - 213 WP_003909442.1 dodecin family protein -
JN144_RS08000 1696623..1697021 + 399 WP_003900351.1 hypothetical protein -
JN144_RS08005 1697066..1698094 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T189304 WP_003407593.1 NZ_CP069069:1692786-1693103 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T189304 NZ_CP090725:c1637171-1636752 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCCTCATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT189304 WP_003900349.1 NZ_CP069069:1692487-1692789 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT189304 NZ_CP090725:c1637407-1637168 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCCAACCGATCCGATCAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References