Detailed information of TA system TA189299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242958..1243526
• Replicon: chromosome
• Accession: NZ_CP069069
• Organism: Mycobacterium tuberculosis strain N1176

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JN144_RS05975
• Protein ID: WP_003909358.1
• Coordinates: 1243152..1243526 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN144_RS05970
• Protein ID: WP_003405863.1
• Coordinates: 1242958..1243155 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN144_RS05950	1238999..1239637	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN144_RS05955	1239727..1240734	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN144_RS05960	1240751..1241734	-	984	WP_003898731.1	hypothetical protein	-
JN144_RS05965	1241797..1242870	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN144_RS05970	1242958..1243155	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN144_RS05975	1243152..1243526	+	375	WP_003909358.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN144_RS05980	1243729..1244427	+	699	WP_003909359.1	hypothetical protein	-
JN144_RS05985	1244545..1244730	+	186	WP_003901093.1	hypothetical protein	-
JN144_RS05990	1244657..1244932	-	276	WP_003405867.1	hypothetical protein	-
JN144_RS05995	1245175..1245498	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN144_RS06000	1245513..1246211	-	699	WP_031667523.1	hypothetical protein	-
JN144_RS06005	1246245..1246454	+	210	WP_003911400.1	hypothetical protein	-
JN144_RS06010	1246406..1247176	-	771	Protein_1184	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13391.51 Da
• Isoelectric Point: 4.9046

>T189299 WP_003909358.1 NZ_CP069069:1243152-1243526 [Mycobacterium tuberculosis]
VILVDTSVWIEHLCAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189299 NZ_CP090725:c406741-406442 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAATATTTCGAAGC
CTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATTCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189299 WP_003405863.1 NZ_CP069069:1242958-1243155 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189299 NZ_CP090725:c407062-406745 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Structures

Antitoxin

• AlphaFold DB: G0TH07