Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 756532..757072 Replicon chromosome
Accession NZ_CP069069
Organism Mycobacterium tuberculosis strain N1176

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag JN144_RS03510 Protein ID WP_003403376.1
Coordinates 756532..756840 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag JN144_RS03515 Protein ID WP_003403381.1
Coordinates 756827..757072 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN144_RS03485 751847..753352 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
JN144_RS03490 753433..754443 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
JN144_RS03495 754831..755214 - 384 WP_003403365.1 ribonuclease VapC6 -
JN144_RS03500 755309..755464 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
JN144_RS03505 755540..756256 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
JN144_RS03510 756532..756840 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JN144_RS03515 756827..757072 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
JN144_RS03520 757182..757619 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
JN144_RS03525 757616..757870 - 255 WP_003911263.1 antitoxin VapB7 -
JN144_RS03530 757984..760348 + 2365 Protein_697 arylsulfatase AtsD -
JN144_RS03535 760411..760737 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN144_RS03540 760649..760987 + 339 WP_003403405.1 PIN domain-containing protein -
JN144_RS03545 760984..761157 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T189291 WP_003403376.1 NZ_CP069069:c756840-756532 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T189291 NZ_CP090722:769306-769725 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTTCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
CCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTCGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189291 WP_003403381.1 NZ_CP069069:c757072-756827 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189291 NZ_CP090722:769070-769309 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCAGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TATCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCGAACCGACCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References