Detailed information of TA system TA189285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 698463..699109
• Replicon: chromosome
• Accession: NZ_CP069069
• Organism: Mycobacterium tuberculosis strain N1176

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN144_RS03170
• Protein ID: WP_003403137.1
• Coordinates: 698463..698876 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN144_RS03175
• Protein ID: WP_003403139.1
• Coordinates: 698873..699109 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN144_RS03150	694546..696096	+	1551	WP_003403119.1	MlaD family protein	-
JN144_RS03155	696148..696540	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN144_RS03160	696537..696794	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN144_RS03165	696977..698212	-	1236	WP_003403128.1	ATP-binding protein	-
JN144_RS03170	698463..698876	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN144_RS03175	698873..699109	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN144_RS03180	699213..699719	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN144_RS03185	699833..700363	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN144_RS03190	700347..701042	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN144_RS03195	701165..701476	+	312	WP_003403164.1	hypothetical protein	-
JN144_RS03200	701548..702501	+	954	WP_031667544.1	DUF1259 domain-containing protein	-
JN144_RS03205	702742..703326	+	585	WP_031667543.1	IS607-like element IS1536 family transposase	-
JN144_RS03210	703328..704039	+	712	Protein_636	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T189285 WP_003403137.1 NZ_CP069069:c698876-698463 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T189285 NZ_CP090718:1959992-1960162 [Burkholderia multivorans]
GTGCTCGACGAAGGGGTCTGCGAACTCAAGATCGACTTTGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGT
GGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCAACGCAACGGAAGGACGTCAAGCGCGCCGTGCAGATGGCGCGCGAAC
TGAAGGAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT189285 WP_003403139.1 NZ_CP069069:c699109-698873 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT189285 NZ_CP090718:1960166-1960450 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCAAGGTCGCGCACTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89