Detailed information of TA system TA189276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3835726..3836411
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: JN145_RS18045
• Protein ID: WP_003417998.1
• Coordinates: 3836001..3836411 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN145_RS18040
• Protein ID: WP_003912220.1
• Coordinates: 3835726..3836004 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS18020	3831715..3833316	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
JN145_RS18025	3833333..3834037	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
JN145_RS18030	3834155..3834721	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
JN145_RS18035	3834783..3835670	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
JN145_RS18040	3835726..3836004	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JN145_RS18045	3836001..3836411	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN145_RS18050	3836444..3838180	-	1737	WP_003418002.1	cholesterol oxidase	-
JN145_RS18055	3838236..3839363	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
JN145_RS18060	3839383..3840972	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T189276 WP_003417998.1 NZ_CP069068:3836001-3836411 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T189276 NZ_CP090717:c297079-296729 [Burkholderia multivorans]
ATGGTGAAGCGCGTCAAGTTCGAACGAGGCGATATCGTGCGCGTAAGCCTGAATCCGACTCTCGGTCGTGAGCAGCAGGG
CGACTTTCGTCCGGCGCTGGTGCTCTCCCCGGCAGCGTTCAACGCTTTGGGCGTTGCGCTGGTCGCACCGATCACCCAGG
GTGGCGAATTTGCCCGGTTCGCAGGGTTCGCGGTCCCGCTGTCCGGTGCAGGAACCGCGACGCAGGGCGTCGCGCTGGTG
AACATGGTTCGCATGCTCGACCTGGAGGCGCGCGGCGCTCGCAAGGTCGAACGCGCGCCTGTCGAGGTCGTGGAAGATGC
ACTCGCCCGGCTTCAGACGATCATCGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT189276 WP_003912220.1 NZ_CP069068:3835726-3836004 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT189276 NZ_CP090717:c297324-297073 [Burkholderia multivorans]
ATGGAACTCAAGATTCAAAAATGGGGCAATAGTGCGGCGGTTCGTTTGCCCAGCGTTCTGCTCGAACAGATCAACGCGAC
GGTCGGCAGTTCGCTGAGCGCGGACGTGCGCCCGGATGGCGTGTTGTTGACGCCGGCCCGCCGCAAGTATTCGCTCGATG
ATCTCGTTGCCCAATGCGACACGAAAGCGCCTTTCCCTGCCGACATGGGTGCATGGGGCGAGGTCAAGCCGGTGGGGCGT
GAGGCATGGTGA

Structures

Toxin

• AlphaFold DB: G0TIR9