Detailed information of TA system TA189267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3113679..3114249
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: JN145_RS14840
• Protein ID: WP_003414166.1
• Coordinates: 3113679..3114035 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: JN145_RS14845
• Protein ID: WP_003901465.1
• Coordinates: 3114019..3114249 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS14820	3109131..3110819	-	1689	Protein_2925	alpha/beta hydrolase family protein	-
JN145_RS14825	3110823..3111149	-	327	WP_003414157.1	hypothetical protein	-
JN145_RS14830	3111322..3111909	+	588	WP_031667424.1	DUF3558 family protein	-
JN145_RS14835	3111928..3113577	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
JN145_RS14840	3113679..3114035	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
JN145_RS14845	3114019..3114249	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
JN145_RS14850	3114292..3115335	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
JN145_RS14855	3115334..3115801	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
JN145_RS14860	3115977..3116231	-	255	WP_003917684.1	hypothetical protein	-
JN145_RS14865	3116379..3116783	+	405	WP_003414181.1	hypothetical protein	-
JN145_RS14870	3116780..3116971	+	192	WP_003414184.1	hypothetical protein	-
JN145_RS14875	3117188..3117449	+	262	Protein_2936	transposase	-
JN145_RS14880	3118559..3118816	+	258	WP_003899489.1	hypothetical protein	-
JN145_RS14885	3118921..3119232	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3103714..3127217	23503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T189267 WP_003414166.1 NZ_CP069068:c3114035-3113679 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T189267 NZ_CP090714:2520168-2520545 [Burkholderia multivorans]
ATGGGTATAAATTCATCGATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGCTTCGGATCGGCCGGCGTGCTCGAGGTCGTGGAGTCGGAGGACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGCGCCGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT189267 WP_003901465.1 NZ_CP069068:c3114249-3114019 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT189267 NZ_CP090714:2520545-2520868 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAAATCATCAAGACCCGCAAATGGACGCAGCAGCACGCGGCACGATTGCTCGGCATGA
CGCAACCGAAGTTATCGAACATGCTGCGCGGGCAGTTTCGCGGTGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3