Detailed information of TA system TA189266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3074107..3074794
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JN145_RS14620
• Protein ID: WP_003414064.1
• Coordinates: 3074399..3074794 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN145_RS14615
• Protein ID: WP_003414061.1
• Coordinates: 3074107..3074373 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS14585	3069746..3070648	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN145_RS14590	3070717..3071469	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN145_RS14600	3071713..3071988	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN145_RS14605	3071985..3073607	-	1623	WP_003909739.1	class I SAM-dependent DNA methyltransferase	-
JN145_RS14610	3073694..3074110	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
JN145_RS14615	3074107..3074373	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN145_RS14620	3074399..3074794	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN145_RS14625	3074791..3075060	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN145_RS14630	3075070..3076164	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN145_RS14635	3076161..3076580	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN145_RS14640	3076579..3076653	+	75	Protein_2889	hypothetical protein	-
JN145_RS14645	3076654..3077133	-	480	WP_003414073.1	dihydrofolate reductase	-
JN145_RS14650	3077204..3078004	-	801	WP_003911953.1	thymidylate synthase	-
JN145_RS14655	3078160..3078897	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T189266 WP_003414064.1 NZ_CP069068:c3074794-3074399 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T189266 NZ_CP090714:c2162744-2162331 [Burkholderia multivorans]
ATGAGCGGGGCTGACAAGGCGAAAGTGTTTGTGGACAGCAATGTCGTGCTGTATCTGCTGTCCGGCGACGCCGCGAAGGC
GGATCGTGCGGAGGCTCTGTTGCGGCGCCGGCCGACGATCAGCGTTCAGGTGCTGAACGAAGTCACGAATGTCTGCGTGC
GTAAGCTCGGGATGGCGTGGCGTGAGGTCGATCAGTTCCTGGAACTGGTGCGGCAGTTTTGCGTGGTCGCGCCGCTGACA
ATCGAGATGCATGATTTGGCGCGGCGCATCGCCGAGCGCTATGGCCTGGCGTTCTATGATGCGTGCATCGTAGCAGCCGC
GTCGATCGAAGGATGCCACGCCCTCTACACGGAAGACATGCATCACGGATTGATCATCGAAGAGAGCTTGACGTTGCGTA
ACCCTTTCGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189266 WP_003414061.1 NZ_CP069068:c3074373-3074107 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189266 NZ_CP090714:c2162964-2162731 [Burkholderia multivorans]
ATGCAGGTTGCTAAATGGGGCAATAGCCTTGCCGTCCGCCTTCCGTCGAGCCTGGTCGAAGCGCTCGAGCTGCGTGAGGG
GGACGATATCGAGATCGTCATAGACGCGCCGCGCACCTTTGCGGTACGCAAGAAACCGGGGGCGGATGAGCTGCTGGAGC
GGTTGCGCCACTTCCGCGGCAAGCTGCCGGCCGATTTCAAGTTCAGCCGGGACGAGGCGAATGAGCGGGGCTGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64