Detailed information of TA system TA189263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2934210..2934924
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: JN145_RS13785
• Protein ID: WP_003413460.1
• Coordinates: 2934484..2934924 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: JN145_RS13780
• Protein ID: WP_003413456.1
• Coordinates: 2934210..2934497 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS13745	2929632..2929877	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN145_RS13750	2929874..2930278	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
JN145_RS13755	2930495..2931115	+	621	WP_003909700.1	DUF4178 domain-containing protein	-
JN145_RS13760	2931126..2931620	+	495	WP_003909701.1	DUF2617 family protein	-
JN145_RS13765	2931617..2932048	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
JN145_RS13770	2932073..2932531	+	459	WP_003413451.1	DUF350 domain-containing protein	-
JN145_RS13775	2932528..2934099	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
JN145_RS13780	2934210..2934497	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
JN145_RS13785	2934484..2934924	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN145_RS13790	2934945..2935700	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
JN145_RS13795	2935833..2936429	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
JN145_RS13800	2936437..2937282	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
JN145_RS13805	2937311..2938210	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
JN145_RS13810	2938338..2939012	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T189263 WP_003413460.1 NZ_CP069068:2934484-2934924 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T189263 NZ_CP090708:c1656778-1656359 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCCTCATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT189263 WP_003413456.1 NZ_CP069068:2934210-2934497 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT189263 NZ_CP090708:c1657014-1656775 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCCAACCGATCCGATCAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8