Detailed information of TA system TA189259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2857793..2858433
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN145_RS13410
• Protein ID: WP_003412970.1
• Coordinates: 2857793..2858212 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN145_RS13415
• Protein ID: WP_003412975.1
• Coordinates: 2858209..2858433 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS13380	2853379..2854101	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN145_RS13385	2854618..2854845	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN145_RS13390	2854842..2855243	+	402	WP_003412963.1	PIN domain-containing protein	-
JN145_RS13395	2855278..2856198	-	921	WP_003412965.1	restriction endonuclease	-
JN145_RS13400	2856539..2856745	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN145_RS13405	2856843..2857792	+	950	Protein_2647	ERCC4 domain-containing protein	-
JN145_RS13410	2857793..2858212	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN145_RS13415	2858209..2858433	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN145_RS13420	2858464..2861307	-	2844	WP_244749709.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN145_RS13425	2861379..2861780	-	402	WP_003412981.1	hypothetical protein	-
JN145_RS13430	2861780..2862250	-	471	WP_244749711.1	transcription antitermination factor NusB	-
JN145_RS13435	2862253..2862816	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189259 WP_003412970.1 NZ_CP069068:c2858212-2857793 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189259 NZ_CP090708:c427541-427224 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189259 WP_003412975.1 NZ_CP069068:c2858433-2858209 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189259 NZ_CP090708:c427220-426921 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAATATTTCGAAGC
CTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATTCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09