Detailed information of TA system TA189253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2259728..2260354
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN145_RS10625
• Protein ID: WP_003410075.1
• Coordinates: 2259956..2260354 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN145_RS10620
• Protein ID: WP_003911750.1
• Coordinates: 2259728..2259955 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS10610	2257767..2258111	-	345	WP_003410065.1	ferredoxin family protein	-
JN145_RS10615	2258300..2259469	-	1170	WP_003899126.1	ATP-binding protein	-
JN145_RS10620	2259728..2259955	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN145_RS10625	2259956..2260354	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JN145_RS10630	2260537..2260968	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN145_RS10635	2261069..2261503	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN145_RS10640	2261940..2262119	-	180	Protein_2103	hypothetical protein	-
JN145_RS10645	2262207..2262827	+	621	WP_031667492.1	transposase	-
JN145_RS10650	2262781..2263371	+	591	WP_003899131.1	IS110 family transposase	-
JN145_RS10655	2263499..2264755	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T189253 WP_003410075.1 NZ_CP069068:2259956-2260354 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T189253 NZ_CP090705:1726799-1727377 [Burkholderia multivorans]
ATGAACCCTGACACGATCCGCGTACGGCTCGATTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCGGCCG
CACGTCGTCCGGTCGCCGCGAAAGCACCGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTAGTGTTCGCCGACGCAC
TGCCCGGCATCGACACGCTGAAGCTGCTCAAGCTGTGCGTCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATACCG
GCGACCGAGCAAGCCGCGCATCCGGACAAGAACATGCACGAACGCGACGACCTGCTCACGTTGACGGCCGCACTCGATCC
CGTGCTGCGTAACGAGCTCGTCGCACTGTGGGACGAATACGACGCGGCCGCGTCGCCCGAAGCGCGCGCCGCGAAGGCGC
TCGACAAGCTCGAGACGATTCTGCAGCACACGCAGGGCGCCAACCCGCCCGACTTCGACTACGCGTTCAATCTCGACTAC
GGCCGGCGCTATACGGATGCGGCACCGCTGTTCAGCGCGATCCGCGCGATCGTCGATGCGGACACGCAGCGCAGGATCGA
CGCGCAAGCACAAACGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT189253 WP_003911750.1 NZ_CP069068:2259728-2259955 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT189253 NZ_CP090705:1726107-1726664 [Burkholderia multivorans]
ATGCCCGATTCGCCGCTTCATACGCCGCCGCTGGGCCCGCAGCTGAAACGCTGGCGCCTGCTGCATCGCGTCAAGCAAAG
CCATGCCGCCGAGCTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCGTCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGCCGAACGCCTGCTCGCCGCGCACCTCGACTCCGCGGGCGACCATGCGCTCGCGCGGCTGGTCGCAGGG
AGCGCGGGCCGCATGCATCTCGTCTGCGATCTGACGCACCGGCTGCTCGCATGCTCGCCGTCTCGCGCAGCCGAGTTCTC
GCAGCCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACAGAAGAGATCGTCCGGATGGAAACCGCACTGG
ACACACTCGGCTGGCACGATTGCGCAGGACCGCCGAGCGTCGAATTCGACACCGGCACGAACGCGTCGCGCGTCGTGCCG
ATCCACCGCAGCCGATGCCGCTGGACGCGGATCATGCTGTCGGACGGATCGGCGGCACGGCTCGTCGAAACGCTGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8