Detailed information of TA system TA189241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1547448..1548498
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: G0TIH2
• Locus tag: JN145_RS07325
• Protein ID: WP_003407164.1
• Coordinates: 1547448..1548269 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: JN145_RS07330
• Protein ID: WP_003407167.1
• Coordinates: 1548262..1548498 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS07305	1543006..1543668	-	663	WP_003407152.1	membrane protein	-
JN145_RS07310	1543665..1544450	-	786	WP_003407157.1	membrane protein	-
JN145_RS07315	1544741..1546702	-	1962	WP_003407159.1	sigma-F factor regulator	-
JN145_RS07320	1546841..1547227	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
JN145_RS07325	1547448..1548269	+	822	WP_003407164.1	GTP pyrophosphokinase family protein	Toxin
JN145_RS07330	1548262..1548498	+	237	WP_003407167.1	hypothetical protein	Antitoxin
JN145_RS07335	1548570..1549775	-	1206	WP_003918234.1	serine hydrolase domain-containing protein	-
JN145_RS07340	1550078..1550863	+	786	WP_003407180.1	lipoprotein LprF	-
JN145_RS07345	1551059..1552528	+	1470	WP_003900326.1	cytochrome b5 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30100.10 Da
• Isoelectric Point: 5.8187

>T189241 WP_003407164.1 NZ_CP069068:1547448-1548269 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADLLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T189241 NZ_CP090702:c1307643-1307068 [Burkholderia multivorans]
ATGACGCATTCGCCATTTACGGCGATCTACGATGCCTGTGTGCTTTATCCCGCGCCGCTTCGCGATTTCCTGATGCGGCT
GGCGTTGAGTGGATGTTTCCGGGCGCGGTGGAGCGCACATATTACCGACGAGTGGAAGCGCAATCTGTTGGACAACCGTC
CGGATTTGACAGCGGAACAGCTTGATCGAACCTCAAGTTTGATGGATCGAGCGATACCGGATGCCGTCGTGACCGGTTAT
GAATCGTTGATTGCCGGGCTTGTTCTCCCGGATGCCGACGATCGTCACGTGCTTGCCGCTGCAATCCGTTGCAACGCCAG
CGTGATCGTGACGTTCAATCGAAAGGACTTTCCGGAAGAGGCGTTGTTGCCGTACGGCATCGAGGCCCAGCATCCAGACG
CGTTCGTCGATGATCTTTTCGACCTCGAACCCGCGGTGGTCGTTGCGGCCGCGCGACGACGGCGAGAGGCGCTTAAACAA
CCACCGTTGACTGCAGACCGGTACCTCGAGATCTTGCGACGACAAGGCATGGTGCAGACGTGTCAGTCGCTCGACGCCTA
TCGAGCACTTCTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT189241 WP_003407167.1 NZ_CP069068:1548262-1548498 [Mycobacterium tuberculosis]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT189241 NZ_CP090702:c1308107-1307640 [Burkholderia multivorans]
ATGCATCACGCCACGACACCCGACATGATCTTGCCCGTCGAGCGCGAGATGCAGGCGGCCGTCGAGGGGCAACGCATGCT
GGCGGCTTATCTCGCAACACGGACCGATACGCAGCGCATTCAGATTTTTGATGACGAGAACCGAGCCCATCAGGTCGAGC
TGCCGACGTCCGCACTTCGTCTGCTGGTGGATATTCTCGCGGAATTGGCGGATGGCAACGCTGTCAAGGTTGTGCCGGTT
CATGCGGAATTGACGACGCAGGAAGCGGCAGATCTGCTCAATGTCTCCCGCCCGCATCTGATCAAGCTGCTTGAGTCGAA
TGCATTGCCCTATCACCGGACGGGCAAGCATCGGCGCGTGCGGTTTTCGGATCTGATGCGCTACAAGGCTGAGCGCGACC
AAGAGAGTGCTGACGCAATGGAAGAACTGAGCAAGCAGGCACAGGATCTGCGGATGGGCTATGAATGA

Structures

Toxin

• AlphaFold DB: G0TIH2

Antitoxin

• AlphaFold DB: A0A7U4BTV8