Detailed information of TA system TA189238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1243272..1243840
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JN145_RS05965
• Protein ID: WP_003909358.1
• Coordinates: 1243466..1243840 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN145_RS05960
• Protein ID: WP_003405863.1
• Coordinates: 1243272..1243469 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS05940	1239313..1239951	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN145_RS05945	1240041..1241048	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN145_RS05950	1241065..1242048	-	984	WP_003898731.1	hypothetical protein	-
JN145_RS05955	1242111..1243184	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN145_RS05960	1243272..1243469	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN145_RS05965	1243466..1243840	+	375	WP_003909358.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN145_RS05970	1244043..1244741	+	699	WP_003909359.1	hypothetical protein	-
JN145_RS05975	1244859..1245044	+	186	WP_003901093.1	hypothetical protein	-
JN145_RS05980	1244971..1245246	-	276	WP_003405867.1	hypothetical protein	-
JN145_RS05985	1245489..1245812	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN145_RS05990	1245827..1246525	-	699	WP_244749833.1	guanylate cyclase	-
JN145_RS05995	1246559..1246768	+	210	WP_003911400.1	hypothetical protein	-
JN145_RS06000	1246720..1247490	-	771	Protein_1182	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13391.51 Da
• Isoelectric Point: 4.9046

>T189238 WP_003909358.1 NZ_CP069068:1243466-1243840 [Mycobacterium tuberculosis]
VILVDTSVWIEHLCAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189238 NZ_CP090702:c621257-620679 [Burkholderia multivorans]
ATGAACCCCGACACGATCCGCGCACGGCTCGATTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCGGCCG
CACGTCGTCCGGTCGCCGCGAAAGCACCGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCGCCGACGCAC
TGCCCGGCATCGACACGCTGAAGCTGCTCAAGCTGTGCGTCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATACCG
GCGCCCGAGCAAGCCGCGCATCCGGACAAGAACATGCACGAACGCGACGACCTGCTCACGTTGACGGCCGCACTCGATCC
CGTGCTGCGTAACGAGCTCGTCGCACTGTGGGACGAATACGACGCGGCCGCGTCGCCCGAAGCGCGCGCCGCGAAGGCGC
TCGACAAGCTCGAGACGATTCTGCAGCACACGCAGGGCGCCAACCCGCCCGACTTCGACTACGCGTTCAATCTCGACTAC
GGCCGGCGCTATACGGATGCGGCACCGCTGTTCAGCGCGATCCGCGCGATCGTCGATGCGGACACGCAGCGCAGGATCGA
CGCGCAAGCACAAACGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189238 WP_003405863.1 NZ_CP069068:1243272-1243469 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189238 NZ_CP090702:c621949-621392 [Burkholderia multivorans]
ATGCCCGATTCGCCGCTTCATACGCCGCCGCTGGGCCCGCAGCTGAAACGCTGGCGCCTGCTGCATCGCGTCAAGCAAAG
CCATGCCGCCGAGCTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCGTCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGACGAACGCCTGCTCGCCGCGCACCTCGACTCCGCGGGCGACCATGCGCTCGCGCGGCTGGTCGCAGGG
AGCGCGGGCCGCATGCATCTCGTCTGCGATCTGACGCACCGGCTGCTCGCATGCTCGCCGTCTCGCGCAGCCGAGTTCTC
GCAGCCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACAGAAGAGATCGTCCGGATGGAAACCGCACTGG
ACACACTCGGCTGGCACGATTGCGCAGGACCGCCGAGCGTCGAATTCGACACCGGCACGAACGCGTCGCGCGTCGTGCCG
ATCCACCGCAGCCGATGCCGCTGGACGCGGATCATGCTGTCGGACGGATCGGCGGCACGGCTCGTCGAAACGCTGTAG

Structures

Antitoxin

• AlphaFold DB: G0TH07