Detailed information of TA system TA189234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016117..1016736
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN145_RS04830
• Protein ID: WP_003404726.1
• Coordinates: 1016302..1016736 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: JN145_RS04825
• Protein ID: WP_003404724.1
• Coordinates: 1016117..1016296 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS04810	1011590..1013170	+	1581	WP_003909313.1	serine hydrolase	-
JN145_RS04815	1013167..1015560	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JN145_RS04820	1015833..1015991	+	159	WP_003404720.1	hypothetical protein	-
JN145_RS04825	1016117..1016296	+	180	WP_003404724.1	antitoxin	Antitoxin
JN145_RS04830	1016302..1016736	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN145_RS04835	1016834..1017607	+	774	WP_003404735.1	VOC family protein	-
JN145_RS04840	1017672..1018121	+	450	WP_003404738.1	hypothetical protein	-
JN145_RS04845	1018256..1018486	-	231	WP_003898642.1	hypothetical protein	-
JN145_RS04850	1018653..1020161	-	1509	WP_244749819.1	carotenoid oxygenase family protein	-
JN145_RS04855	1020163..1021401	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T189234 WP_003404726.1 NZ_CP069068:1016302-1016736 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T189234 NZ_CP090699:c1856061-1855645 [Burkholderia cepacia]
GTGACCACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCGCCGGTAGTGCTGTCGCGGCT
GCAATCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCCATCACTTATGCCGAAATGCGGTTCGGCGCAGTCGGCA
GGAAGGCTTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTCTCGCGGCTCGATGGCGTGCTGTCGTGGGATACGGCG
GCCGTCGATGCAACGGCCGCGATTCGCGCCGATCTCGCCGCGCGCGGCATGCCGATCGGCGCGAACGATGCGTCGATCGC
CGGGCATGCGATCGCGGCCGGTGCGGTGCTCGTCACGAACAACGGGCGGGAGTTCGGCCGGGTGGCCGGGTTGTCGCTCG
AAGACTGGGCAGCCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT189234 WP_003404724.1 NZ_CP069068:1016117-1016296 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT189234 NZ_CP090699:c1856318-1856058 [Burkholderia cepacia]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
GGAACTCGAGATCCGTCGCGAGGGCGACACGCTGCTGCTGCGGCCCGTGCGGCCCACGTGGCTCTCGTTCGCGAGCGAAC
CGCTGGCCGACGCCGATTTCCTCGCCGAACGCCCGGCCGTGATCGAGTCCGGCCGCTTCGACCTTTCCGGCGGCGCCGCG
CCGACGGAGTCCGGCCAGTGA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5