Detailed information of TA system TA189225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 705231..705874
• Replicon: chromosome
• Accession: NZ_CP069068
• Organism: Mycobacterium tuberculosis strain N1272

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: JN145_RS03215
• Protein ID: WP_003403187.1
• Coordinates: 705473..705874 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: JN145_RS03210
• Protein ID: WP_003403184.1
• Coordinates: 705231..705476 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN145_RS03175	700507..701037	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN145_RS03180	701021..701716	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN145_RS03185	701839..702150	+	312	WP_003403164.1	hypothetical protein	-
JN145_RS03190	702222..703175	+	954	WP_244749805.1	DUF1259 domain-containing protein	-
JN145_RS03195	703416..704000	+	585	WP_031667543.1	IS607-like element IS1536 family transposase	-
JN145_RS03200	704002..704713	+	712	Protein_634	IS607 family element RNA-guided endonuclease TnpB	-
JN145_RS03205	704716..705186	+	471	WP_003898523.1	hypothetical protein	-
JN145_RS03210	705231..705476	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN145_RS03215	705473..705874	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN145_RS03220	705865..706044	+	180	Protein_638	hypothetical protein	-
JN145_RS03225	706462..706659	-	198	WP_003403191.1	hypothetical protein	-
JN145_RS03230	706739..707896	-	1158	WP_003403193.1	hypothetical protein	-
JN145_RS03235	707948..708169	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
JN145_RS03240	708311..708916	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T189225 WP_003403187.1 NZ_CP069068:705473-705874 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T189225 NZ_CP090698:1013087-1013506 [Burkholderia cepacia]
ATGATCCTTGTTGATACGAATGTCATTTCCGAACCGCTACGACGCGAGCCGAACGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAATGTCGAAACCTTGTTTCTGGCTGCGATCAGTCTCGCGGAAATACGATTGGGCGTGGCTGTATTGCCGGAAGGAC
GCCGGCGAGAGTGGCTGCATCAGAGCATCGAGCAGCGCGTGCTGCCATTGTTTCGCGGACGAATCCTGCCATTCGACGAT
GCTGCGAGCAAAGCGTATGCGAGCCTTCGCGCACGCGCTCGTGCCGCAGGTGTCGCAATTGCACCATCCGATGGTTTTAT
CGCCGGCGCGGCCGAAGCGAACGGCCTGATCGTCGCCACGCGCGATGTCACGCCGTTCGAGGCAATGGGAATTCGCGTAA
TCGACCCGTGGGCGCGCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT189225 WP_003403184.1 NZ_CP069068:705231-705476 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT189225 NZ_CP090698:1012851-1013090 [Burkholderia cepacia]
ATGCCGGTGATCACCGTCCGAAATCTGTCCGATGAAGTGCATCGAGCACTTCGCATTCGCGCGGCCCAACATGGGCGCAG
CACCGAAGCCGAGGTGCGCGACATTCTCGAACAGGCCGTTCTTCCGGGCGGGCGGCTCAAGCTGGGAACGATGCTGGCGG
CAATCGGGCAGGAGGCCGGCGGATTCGATTTCGACGCCCAGCGCGACAAGACGCCTGCCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91