Detailed information of TA system TA189213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3792846..3793375
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN146_RS17865
• Protein ID: WP_003417760.1
• Coordinates: 3793118..3793375 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN146_RS17860
• Protein ID: WP_003417757.1
• Coordinates: 3792846..3793121 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS17825	3789419..3790108	-	690	WP_003917774.1	BBE domain-containing protein	-
JN146_RS17830	3790295..3790666	-	372	WP_003417739.1	FAD-binding protein	-
JN146_RS17835	3790564..3790782	-	219	WP_157132559.1	hypothetical protein	-
JN146_RS17840	3790809..3791069	-	261	WP_003417741.1	hypothetical protein	-
JN146_RS17845	3791184..3791573	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN146_RS17850	3791587..3791880	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN146_RS17855	3791877..3792722	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN146_RS17860	3792846..3793121	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN146_RS17865	3793118..3793375	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN146_RS17870	3793417..3794607	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN146_RS17875	3794724..3795092	+	369	WP_003417765.1	FHA domain-containing protein	-
JN146_RS17880	3795089..3795640	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN146_RS17885	3795647..3796228	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN146_RS17890	3796209..3796577	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN146_RS17895	3796555..3796947	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189213 WP_003417760.1 NZ_CP069067:3793118-3793375 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189213 NZ_CP090694:1965604-1965903 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
CTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189213 WP_003417757.1 NZ_CP069067:3792846-3793121 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189213 NZ_CP090694:1965283-1965600 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94