Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3150664..3151268 Replicon chromosome
Accession NZ_CP069067
Organism Mycobacterium tuberculosis strain N1268

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN146_RS15020 Protein ID WP_003414492.1
Coordinates 3150664..3151056 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN146_RS15025 Protein ID WP_003414495.1
Coordinates 3151053..3151268 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN146_RS14990 3145814..3146602 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JN146_RS14995 3146936..3147481 - 546 WP_244748585.1 DUF1802 family protein -
JN146_RS15000 3147753..3148637 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN146_RS15005 3148640..3149527 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN146_RS15010 3149832..3150377 - 546 WP_003899500.1 DUF1802 family protein -
JN146_RS15015 3150374..3150643 - 270 WP_003414489.1 DUF2277 family protein -
JN146_RS15020 3150664..3151056 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN146_RS15025 3151053..3151268 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN146_RS15030 3151315..3152064 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN146_RS15035 3152143..3153225 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN146_RS15040 3153218..3154528 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JN146_RS15045 3154531..3155358 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN146_RS15050 3155355..3156266 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189208 WP_003414492.1 NZ_CP069067:c3151056-3150664 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189208 NZ_CP090694:749159-749614 [Burkholderia multivorans]
ATGATCCTTGTTGATACAAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
GCAAAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGAC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCTTGGGCGTCGCAGGGCGCGCCGGTGCGCCAGGGCATCGCGTCGAAATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189208 WP_003414495.1 NZ_CP069067:c3151268-3151053 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189208 NZ_CP090694:748923-749162 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCACTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATTCAGCGTGACAAGACGCTAACCGATCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References