Detailed information of TA system TA189207

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 3147753..3149527
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: P71626
• Locus tag: JN146_RS15000
• Protein ID: WP_003414409.1
• Coordinates: 3147753..3148637 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: P71625
• Locus tag: JN146_RS15005
• Protein ID: WP_003414414.1
• Coordinates: 3148640..3149527 (-)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS14980	3143008..3143382	-	375	WP_003414296.1	type III-A CRISPR-associated protein Csm2	-
JN146_RS14985	3143379..3145817	-	2439	WP_031667895.1	type III-A CRISPR-associated protein Cas10/Csm1	-
JN146_RS14990	3145814..3146602	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
JN146_RS14995	3146936..3147481	-	546	WP_244748585.1	DUF1802 family protein	-
JN146_RS15000	3147753..3148637	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JN146_RS15005	3148640..3149527	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
JN146_RS15010	3149832..3150377	-	546	WP_003899500.1	DUF1802 family protein	-
JN146_RS15015	3150374..3150643	-	270	WP_003414489.1	DUF2277 family protein	-
JN146_RS15020	3150664..3151056	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	-
JN146_RS15025	3151053..3151268	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JN146_RS15030	3151315..3152064	+	750	WP_003414497.1	enoyl-CoA hydratase	-
JN146_RS15035	3152143..3153225	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• AbiEi antitoxin C-terminal domain (84-237)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33056.72 Da
• Isoelectric Point: 7.1520

>T189207 WP_003414409.1 NZ_CP069067:c3148637-3147753 [Mycobacterium tuberculosis]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Nucleotide

• Length: 885 bp

>T189207 NZ_CP090694:c585655-585044 [Burkholderia multivorans]
ATGAACCCCGACACGATCCGCGCACGGCTCGATTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCGGCCG
CACGTCGTCCGGTCGCCGCGAAAGCACCGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCGCCGACGCAC
TGCCCGGCATCGACACACTGAAGCTGCTCAAGCTGTGCGTCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATACCG
GCGACCGAGCAAGCCGCGCATCCGGAGAAGAACATGCACGAACGCGACGACCTGCTCACGCTGACGGCCGCACTCGATCC
CGTGCTGCGTAACGAGCTCGTCGCACTGTGGGACGAATACGACGCGGCCGCGTCGCCCGAAGCGCGCGCCGCGAAGGCGC
TCGACAAGCTCGAGACAATTCTGCAGCACGCGCAGGGCGCCAATCCGCCCGACTTCGACTACGCGTTCAATCTCGGCTAC
GGCCGGCGCTATACGGACGCGGCACCGCTATTCAGCGCGATCCGGGCGATCGTCGATGCGGACACGCAGCGCAGGATCGA
CGCGCAAGCACAAACGTCATCGCAAACACAAACGCACACGCACCGCCCATAA

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32315.19 Da
• Isoelectric Point: 9.4723

>AT189207 WP_003414414.1 NZ_CP069067:c3149527-3148640 [Mycobacterium tuberculosis]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Nucleotide

• Length: 888 bp

>AT189207 NZ_CP090694:c586334-585777 [Burkholderia multivorans]
ATGCCCGATTCGCCGCTTCACACGCCGCCGCTGGGCCCGCAGCTAAACCGTTGGCGTCTGCTGCATCGCGTCAAGCAAAG
CCATGCCGCCGCGCTATTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCCTCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGCCGAACGCCTGCTCGCCGCGCGCCTCGACTCCGCGGGCGACCATGCGCTCGCGCGGCTGGTCGCAGGC
AGCGCGGGCCGCATGCATCTCGTCTGCGATCTGACGCACCGGCTGCTTGCATGCTCGCCGTCTCGCGCAGCCGAGTTCTC
GCAGCCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACAGAAGAAATCGTCCGGATGGAAACCGCACTGG
ACACACTCGGCTGGCACGACTGCGCAGGACCGCCGAGCGTCGAATTCGACACCGGCACGAACGCGTCGCGCGTCGTGCCG
ATCCACCGCAGCCGATGCCGCTGGACGCGCATCATGCTGTCGGACGGGTCGGCGGCACGGCTTGTCGAAACGCTGTAG

Structures

Toxin

• PDB: 6Y56

Antitoxin

• AlphaFold DB: A0A829CBU9