Detailed information of TA system TA189204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3085128..3085807
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN146_RS14650
• Protein ID: WP_003414059.1
• Coordinates: 3085128..3085544 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN146_RS14655
• Protein ID: WP_003414061.1
• Coordinates: 3085541..3085807 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS14625	3081180..3082082	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN146_RS14630	3082151..3082903	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN146_RS14640	3083147..3083422	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN146_RS14645	3083419..3085041	-	1623	WP_003909739.1	class I SAM-dependent DNA methyltransferase	-
JN146_RS14650	3085128..3085544	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN146_RS14655	3085541..3085807	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN146_RS14660	3085833..3086228	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN146_RS14665	3086225..3086494	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN146_RS14670	3086504..3087598	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN146_RS14675	3087595..3088014	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN146_RS14680	3088013..3088087	+	75	Protein_2898	hypothetical protein	-
JN146_RS14685	3088088..3088567	-	480	WP_003414073.1	dihydrofolate reductase	-
JN146_RS14690	3088638..3089438	-	801	WP_003911953.1	thymidylate synthase	-
JN146_RS14695	3089594..3090331	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189204 WP_003414059.1 NZ_CP069067:c3085544-3085128 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189204 NZ_CP090692:c919914-919537 [Burkholderia multivorans]
ATGGGTATAAATTCGTCGATGGTTCCATACACTAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGCGGTTTCGGATCGGCCGGCGTACTCGAGGTCGTGGAGTCGGAGGACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCGACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGGGCAGCCGAAGCACACGCAAATGGAGGAAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189204 WP_003414061.1 NZ_CP069067:c3085807-3085541 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189204 NZ_CP090692:c919537-919214 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTGGGCATGCCCGGCGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAGATCATCAAGACCCGCAAATGGACGCAGCAGCACGCGGCGCGGTTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGACGCGATGTCCAGATCGTCGTAGGACCGGACCGTCATACGGAAGCCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64