Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2885826..2886463 Replicon chromosome
Accession NZ_CP069067
Organism Mycobacterium tuberculosis strain N1268

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN146_RS13560 Protein ID WP_003413180.1
Coordinates 2885826..2886221 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN146_RS13565 Protein ID WP_003413183.1
Coordinates 2886218..2886463 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN146_RS13520 2881911..2882570 + 660 WP_003900846.1 LppA family lipoprotein -
JN146_RS13525 2882567..2883226 + 660 WP_003900847.1 LppA family lipoprotein -
JN146_RS13530 2883223..2883885 + 663 WP_003900848.1 LppA family lipoprotein -
JN146_RS13535 2883882..2884160 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN146_RS13540 2884253..2884666 + 414 WP_003413164.1 PIN domain nuclease -
JN146_RS13545 2884705..2884962 + 258 WP_003909690.1 CopG family transcriptional regulator -
JN146_RS13550 2884959..2885336 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN146_RS13555 2885352..2885726 - 375 WP_003413177.1 hypothetical protein -
JN146_RS13560 2885826..2886221 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN146_RS13565 2886218..2886463 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN146_RS13570 2886874..2887293 - 420 WP_003413190.1 A24 family peptidase -
JN146_RS13575 2887305..2888114 - 810 WP_003413193.1 shikimate dehydrogenase -
JN146_RS13580 2888111..2889364 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN146_RS13585 2889357..2889869 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189201 WP_003413180.1 NZ_CP069067:c2886221-2885826 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189201 NZ_CP090689:1959986-1960156 [Burkholderia multivorans]
GTGCTCGACGAAGGGGTCTGCGAACTCAAGATCGACTTTGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGT
GGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCAACGCAACGGAAGGACGTCAAGCGCGCCGTGCAGATGGCGCGCGAAC
TGAAGGAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189201 WP_003413183.1 NZ_CP069067:c2886463-2886218 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189201 NZ_CP090689:1960160-1960444 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCAAGGTCGCGCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References