Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 2884705..2885336 Replicon chromosome
Accession NZ_CP069067
Organism Mycobacterium tuberculosis strain N1268

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag JN146_RS13550 Protein ID WP_003413174.1
Coordinates 2884959..2885336 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7U8Y8P4
Locus tag JN146_RS13545 Protein ID WP_003909690.1
Coordinates 2884705..2884962 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN146_RS13510 2879963..2880277 + 315 WP_009937839.1 hypothetical protein -
JN146_RS13515 2880573..2881784 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN146_RS13520 2881911..2882570 + 660 WP_003900846.1 LppA family lipoprotein -
JN146_RS13525 2882567..2883226 + 660 WP_003900847.1 LppA family lipoprotein -
JN146_RS13530 2883223..2883885 + 663 WP_003900848.1 LppA family lipoprotein -
JN146_RS13535 2883882..2884160 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN146_RS13540 2884253..2884666 + 414 WP_003413164.1 PIN domain nuclease -
JN146_RS13545 2884705..2884962 + 258 WP_003909690.1 CopG family transcriptional regulator Antitoxin
JN146_RS13550 2884959..2885336 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
JN146_RS13555 2885352..2885726 - 375 WP_003413177.1 hypothetical protein -
JN146_RS13560 2885826..2886221 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
JN146_RS13565 2886218..2886463 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
JN146_RS13570 2886874..2887293 - 420 WP_003413190.1 A24 family peptidase -
JN146_RS13575 2887305..2888114 - 810 WP_003413193.1 shikimate dehydrogenase -
JN146_RS13580 2888111..2889364 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN146_RS13585 2889357..2889869 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T189200 WP_003413174.1 NZ_CP069067:2884959-2885336 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T189200 NZ_CP090688:2120818-2121168 [Burkholderia multivorans]
ATGGTGAAGCGCGTCAAGTTCGAACGAGGCGATATCGTGCGCGTAAGCCTGAATCCGACTCTCGGTCGTGAGCAGCAGGG
CGACTTTCGTCCGGCGCTGGTGCTCTCCCCGGCAGCGTTCAACGCTTTGGGCGTTGCGCTGGTCGCACCGATCACCCAGG
GTGGCGAATTTGCCCGGTTCGCAGGGTTCGCGGTCCCGCTGTCCGGTGCAGGAACCGCGACGCAGGGCGTCGCGCTGGTG
AACATGGTTCGCATGCTCGACCTGGAGGCGCGCGGCGCTCGCAAGGTCGAACGCGCGCCTGTCGAGGTCGTGGAAGATGC
ACTCGCCCGGCTTCAGACGATCATCGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9511.67 Da        Isoelectric Point: 10.2895

>AT189200 WP_003909690.1 NZ_CP069067:2884705-2884962 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRDSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT189200 NZ_CP090688:2120573-2120824 [Burkholderia multivorans]
ATGGAACTCAAGATTCAAAAATGGGGCAATAGTGCGGCGGTTCGTTTGCCCAGCGTTCTGCTCGAACAGATCAACGCGAC
GGTCGGCAGTTCGCTGAGCGCGGACGTGCGCCCGGATGGCGTGTTGTTGACGCCGGCCCGCCGCAAGTATTCGCTCGATG
ATCTCGTTGCCCAATGCGACACGAAAGCGCCTTTCCCTGCCGACATGGGTGCATGGGGCGAGGTCAAGCCGGTGGGGCGT
GAGGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8Y8P4

References