Detailed information of TA system TA189200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2884705..2885336
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: JN146_RS13550
• Protein ID: WP_003413174.1
• Coordinates: 2884959..2885336 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7U8Y8P4
• Locus tag: JN146_RS13545
• Protein ID: WP_003909690.1
• Coordinates: 2884705..2884962 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS13510	2879963..2880277	+	315	WP_009937839.1	hypothetical protein	-
JN146_RS13515	2880573..2881784	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN146_RS13520	2881911..2882570	+	660	WP_003900846.1	LppA family lipoprotein	-
JN146_RS13525	2882567..2883226	+	660	WP_003900847.1	LppA family lipoprotein	-
JN146_RS13530	2883223..2883885	+	663	WP_003900848.1	LppA family lipoprotein	-
JN146_RS13535	2883882..2884160	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN146_RS13540	2884253..2884666	+	414	WP_003413164.1	PIN domain nuclease	-
JN146_RS13545	2884705..2884962	+	258	WP_003909690.1	CopG family transcriptional regulator	Antitoxin
JN146_RS13550	2884959..2885336	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN146_RS13555	2885352..2885726	-	375	WP_003413177.1	hypothetical protein	-
JN146_RS13560	2885826..2886221	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN146_RS13565	2886218..2886463	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN146_RS13570	2886874..2887293	-	420	WP_003413190.1	A24 family peptidase	-
JN146_RS13575	2887305..2888114	-	810	WP_003413193.1	shikimate dehydrogenase	-
JN146_RS13580	2888111..2889364	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN146_RS13585	2889357..2889869	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T189200 WP_003413174.1 NZ_CP069067:2884959-2885336 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T189200 NZ_CP090688:2120818-2121168 [Burkholderia multivorans]
ATGGTGAAGCGCGTCAAGTTCGAACGAGGCGATATCGTGCGCGTAAGCCTGAATCCGACTCTCGGTCGTGAGCAGCAGGG
CGACTTTCGTCCGGCGCTGGTGCTCTCCCCGGCAGCGTTCAACGCTTTGGGCGTTGCGCTGGTCGCACCGATCACCCAGG
GTGGCGAATTTGCCCGGTTCGCAGGGTTCGCGGTCCCGCTGTCCGGTGCAGGAACCGCGACGCAGGGCGTCGCGCTGGTG
AACATGGTTCGCATGCTCGACCTGGAGGCGCGCGGCGCTCGCAAGGTCGAACGCGCGCCTGTCGAGGTCGTGGAAGATGC
ACTCGCCCGGCTTCAGACGATCATCGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9511.67 Da
• Isoelectric Point: 10.2895

>AT189200 WP_003909690.1 NZ_CP069067:2884705-2884962 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRDSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189200 NZ_CP090688:2120573-2120824 [Burkholderia multivorans]
ATGGAACTCAAGATTCAAAAATGGGGCAATAGTGCGGCGGTTCGTTTGCCCAGCGTTCTGCTCGAACAGATCAACGCGAC
GGTCGGCAGTTCGCTGAGCGCGGACGTGCGCCCGGATGGCGTGTTGTTGACGCCGGCCCGCCGCAAGTATTCGCTCGATG
ATCTCGTTGCCCAATGCGACACGAAAGCGCCTTTCCCTGCCGACATGGGTGCATGGGGCGAGGTCAAGCCGGTGGGGCGT
GAGGCATGGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A7U8Y8P4