Detailed information of TA system TA189198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2869710..2870350
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN146_RS13450
• Protein ID: WP_003412970.1
• Coordinates: 2869710..2870129 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN146_RS13455
• Protein ID: WP_003412975.1
• Coordinates: 2870126..2870350 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS13420	2865296..2866018	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN146_RS13425	2866535..2866762	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN146_RS13430	2866759..2867160	+	402	WP_003412963.1	PIN domain-containing protein	-
JN146_RS13435	2867195..2868115	-	921	WP_003412965.1	restriction endonuclease	-
JN146_RS13440	2868456..2868662	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN146_RS13445	2868760..2869709	+	950	Protein_2656	ERCC4 domain-containing protein	-
JN146_RS13450	2869710..2870129	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN146_RS13455	2870126..2870350	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN146_RS13460	2870381..2873224	-	2844	WP_003909688.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN146_RS13465	2873296..2873697	-	402	WP_003412981.1	hypothetical protein	-
JN146_RS13470	2873697..2874167	-	471	WP_003412985.1	transcription antitermination factor NusB	-
JN146_RS13475	2874170..2874733	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189198 WP_003412970.1 NZ_CP069067:c2870129-2869710 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189198 NZ_CP090688:c1630596-1630141 [Burkholderia multivorans]
ATGATCCTTGTTGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
ACAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCGCAGGGCGCGCCGGTGCGCCAGGGCATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189198 WP_003412975.1 NZ_CP069067:c2870350-2870126 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189198 NZ_CP090688:c1630832-1630593 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCACTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGTGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGACGGGAGGCAGGCGGTGTCGATTTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09