Detailed information of TA system TA189194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 2340764..2341400
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P0CL62
• Locus tag: JN146_RS10945
• Protein ID: WP_003410654.1
• Coordinates: 2340990..2341400 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: JN146_RS10940
• Protein ID: WP_003410651.1
• Coordinates: 2340764..2340997 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS10925	2336212..2336613	+	402	WP_009937711.1	metal ABC transporter permease	-
JN146_RS10930	2336614..2337018	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
JN146_RS10935	2337102..2340686	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
JN146_RS10940	2340764..2340997	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
JN146_RS10945	2340990..2341400	+	411	WP_003410654.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
JN146_RS10950	2341384..2342475	+	1092	WP_003900454.1	precorrin-3B synthase	-
JN146_RS10955	2342485..2343111	+	627	WP_003410658.1	precorrin-8X methylmutase	-
JN146_RS10960	2343108..2344634	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
JN146_RS10965	2344580..2345803	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14235.42 Da
• Isoelectric Point: 10.6228

>T189194 WP_003410654.1 NZ_CP069067:2340990-2341400 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T189194 NZ_CP090688:1046458-1047033 [Burkholderia multivorans]
ATGACGCATTCGCCATTTACGGCGATCTACGATGCCTGTGTGCTTTATCCCGCGCCGCTTCGCGATTTCCTGATGCGGCT
GGCGTTGAGTGGATGTTTCCGGGCGCGGTGGAGCGCACATATTACCGACGAGTGGAAGCGCAATCTGTTGGACAACCGTC
CGGATTTGACAGCGGAACAGCTTGATCGAACGTCAAGTTTGATGGATCGAGCGATACCGGATGCCGTCGTGACCGGTTAT
GAATCGTTGATTGCCGGGCTTGTTCTCCCGGATGCCGACGATCGTCACGTGCTTGCCGCTGCAATCCGTTGCAACGCCAG
CGTGATCGTGACGTTCAATCGAAAGGACTTTCCGGAAGAGGCGTTGTTGCCGTACGGCATCGAGGCCCAGCATCCAGACG
AGTTCGTCGATAATCTTTTCGACCTCGAACCCGCGGTGGTCGTTGCGGCCGCGCGACGACAGCGAGAGGCACTTAAACAA
CCACCGTTGACCGCAGACCGGTACCTCGAGATTTTGCGACGACAAGGCATGGTGCAGACGTGTCAGTCGCTCGACGCCTA
TCGAGCACTTCTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT189194 WP_003410651.1 NZ_CP069067:2340764-2340997 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT189194 NZ_CP090688:1045994-1046461 [Burkholderia multivorans]
ATGCATCACGCCACGACACCCGACATGATCTTGCCCGTCGAGCGCGAGATGCAGGCGGCCGTCGAGGGGCAACGCATGCT
GGCGGCTTATCTCGCAACACGGACCGATACGCAGCGCATTCAGATTTTTGATGACGAGAACCGAGCCCATCAGGTCGAGC
TGCCGACGTCCGCACTTCGTCTGCTGGTGGATATTCTCGCGGAATTGGCGGACGGAAACGCTGTCAAGGTTGTGCCGGTT
CATGCGGAATTGACGACGCAGGAAGCGGCAGATCTGCTCAATGTCTCCCGCCCGCATCTGATCAAGCTGCTTGAGTCGAA
TGCATTGCCCTATCACCGGACGGGCAAGCATCGGCGCGTGCGGTTTTCGGATCTGATGCGCTACAAGGCTGAGCGCGACC
AAGAGAGTGCTGACGCAATGGAAGAGCTGGGCAAGCAGGCACAGGCGCTGCGGATGGGCTACGAATGA

Structures

Toxin

• PDB: 7DU4
• PDB: 5WYG
• PDB: 6A6X

Antitoxin

• PDB: 7DU4
• PDB: 6A6X
• AlphaFold DB: A0A7U4BV50