Detailed information of TA system TA189192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2272979..2273605
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN146_RS10655
• Protein ID: WP_003410075.1
• Coordinates: 2273207..2273605 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN146_RS10650
• Protein ID: WP_003911750.1
• Coordinates: 2272979..2273206 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS10640	2271018..2271362	-	345	WP_003410065.1	ferredoxin family protein	-
JN146_RS10645	2271551..2272720	-	1170	WP_003899126.1	ATP-binding protein	-
JN146_RS10650	2272979..2273206	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN146_RS10655	2273207..2273605	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JN146_RS10660	2273788..2274219	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN146_RS10665	2274320..2274754	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN146_RS10670	2275191..2275370	-	180	Protein_2110	hypothetical protein	-
JN146_RS10675	2275458..2276078	+	621	WP_031667492.1	transposase	-
JN146_RS10680	2276032..2276622	+	591	WP_003899131.1	IS110 family transposase	-
JN146_RS10685	2276750..2278006	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T189192 WP_003410075.1 NZ_CP069067:2273207-2273605 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T189192 NZ_CP090688:c425310-425011 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
CTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT189192 WP_003911750.1 NZ_CP069067:2272979-2273206 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT189192 NZ_CP090688:c425631-425314 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCATGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8