Detailed information of TA system TA189186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2214338..2215041
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN146_RS10290
• Protein ID: WP_003409778.1
• Coordinates: 2214338..2214667 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN146_RS10295
• Protein ID: WP_003409780.1
• Coordinates: 2214664..2215041 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS10270	2210721..2211791	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN146_RS10275	2211788..2212303	+	516	WP_003409718.1	flavin reductase family protein	-
JN146_RS10280	2212300..2213361	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN146_RS10285	2213358..2214128	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN146_RS10290	2214338..2214667	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN146_RS10295	2214664..2215041	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN146_RS10300	2215038..2215628	-	591	WP_003909548.1	SEC-C metal-binding domain-containing protein	-
JN146_RS10305	2215683..2217047	+	1365	WP_003909549.1	HNH endonuclease signature motif containing protein	-
JN146_RS10310	2217202..2217654	-	453	WP_003899095.1	lipoprotein	-
JN146_RS10315	2217718..2218119	+	402	WP_003409869.1	hypothetical protein	-
JN146_RS10320	2218112..2218294	-	183	WP_003409870.1	hypothetical protein	-
JN146_RS10325	2218408..2218758	-	351	WP_003409871.1	hypothetical protein	-
JN146_RS10330	2218769..2219671	-	903	WP_003409874.1	hypothetical protein	-
JN146_RS10335	2219692..2219883	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189186 WP_003409778.1 NZ_CP069067:c2214667-2214338 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189186 NZ_CP090686:1963464-1963781 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189186 WP_003409780.1 NZ_CP069067:c2215041-2214664 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189186 NZ_CP090686:1963785-1964084 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8