Detailed information of TA system TA189181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1582969..1583624
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4FAR1
• Locus tag: JN146_RS07475
• Protein ID: WP_003407268.1
• Coordinates: 1582969..1583370 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TIK2
• Locus tag: JN146_RS07480
• Protein ID: WP_003407272.1
• Coordinates: 1583367..1583624 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS07460	1578443..1579828	-	1386	WP_003407260.1	cytochrome P450	-
JN146_RS07465	1579906..1580940	+	1035	WP_003407264.1	AraC family transcriptional regulator	-
JN146_RS07470	1580986..1582714	-	1729	Protein_1472	PE family protein	-
JN146_RS07475	1582969..1583370	-	402	WP_003407268.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN146_RS07480	1583367..1583624	-	258	WP_003407272.1	CopG family transcriptional regulator	Antitoxin
JN146_RS07485	1583707..1584666	-	960	WP_003407276.1	alpha/beta hydrolase	-
JN146_RS07490	1584691..1585653	-	963	WP_003407279.1	alpha/beta hydrolase	-
JN146_RS07495	1585652..1586389	+	738	WP_031647000.1	lysoplasmalogenase	-
JN146_RS07500	1586470..1588437	+	1968	WP_003407285.1	primosomal protein N'	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14894.39 Da
• Isoelectric Point: 11.6897

>T189181 WP_003407268.1 NZ_CP069067:c1583370-1582969 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Nucleotide

• Length: 402 bp

>T189181 NZ_CP090686:c640791-640213 [Burkholderia multivorans]
ATGAACCCCGACACGATCCGCGCACGGCTCGATTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCGGCCG
CACGTCGTCCGGTCGCCGCGAAAGCACCGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCGCCGACGCAC
TGCCCGGCATCGACACGCTGAAGCTGCTCAAGCTGTGCGTCGTCCACGATCTCGGCGAGGCGCTGCACGGCGACATACCG
GCGCCCGAGCAAGCCGCGCATCCGGACAAGAACATGCACGAACGCGACGACCTGCTCACGTTGACGGCCGCACTCGATCC
CGTGCTGCGTAACGAGCTCGTCGCACTGTGGGACGAATACGACGCGGCCGCGTCGCCCGAAGCGCGCGCCGCGAAGGCGC
TCGACAAGCTCGAGACGATTCTGCAGCACACGCAGGGCGCCAACCCGCCCGACTTCGACTACGCGTTCAATCTCGACTAC
GGCCGGCGCTATACGGATGCGGCACCGCTGTTCAGCGCGATCCGCGCGATCGTCGATGCGGACACGCAGCGCAGGATCGA
CGCGCAAGCACAAACGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9443.58 Da
• Isoelectric Point: 6.4631

>AT189181 WP_003407272.1 NZ_CP069067:c1583624-1583367 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Nucleotide

• Length: 258 bp

>AT189181 NZ_CP090686:c641483-640926 [Burkholderia multivorans]
ATGCCCGATTCGCCGCTTCATACGCCGCCGCTGGGCCCGCAGCTGAAACGCTGGCGCCTGCTGCATCGCGTCAAGCAAAG
CCATGCCGCCGAGCTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCGTCCAGCAGATGTCGCCCGACG
AGCGCGCGACGGCCGAACGCCTGCTCGCCGCGCACCTCGACTCCGCGGGCGACCATGCGCTCGCGCGGCTGGTCGCAGGG
AGCGCGGGCCGCATGCATCTCGTCTGCGATCTGACGCACCGGCTGCTCGCATGCTCGCCGTCTCGCGCAGCCGAGTTCTC
GCAGCCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACAGAAGAGATCGTCCGGATGGAAACCGCACTGG
ACACACTCGGCTGGCACGATTGCGCAGGACCGCCGAGCGTCGAATTCGACACCGGCACGAACGCGTCGCGCGTCGTGCCG
ATCCACCGCAGCCGATGCCGCTGGACGCGGATCATGCTGTCGGACGGATCGGCGGCACGGCTCGTCGAAACGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A7U4FAR1

Antitoxin

• AlphaFold DB: G0TIK2