Detailed information of TA system TA189178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1390522..1391210
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: JN146_RS06645
• Protein ID: WP_003406304.1
• Coordinates: 1390779..1391210 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: JN146_RS06640
• Protein ID: WP_003406302.1
• Coordinates: 1390522..1390782 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS06620	1386099..1386923	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
JN146_RS06625	1386928..1388109	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
JN146_RS06630	1388186..1389286	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
JN146_RS06635	1389457..1390446	+	990	WP_003909381.1	malate dehydrogenase	-
JN146_RS06640	1390522..1390782	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
JN146_RS06645	1390779..1391210	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN146_RS06650	1391233..1392908	-	1676	Protein_1314	PE family protein	-
JN146_RS06655	1393088..1393948	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
JN146_RS06660	1394029..1394859	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
JN146_RS06665	1394916..1395209	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
JN146_RS06670	1395206..1395475	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189178 WP_003406304.1 NZ_CP069067:1390779-1391210 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189178 NZ_CP090682:c1507517-1507233 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCAAGGTCGCGCACTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189178 WP_003406302.1 NZ_CP069067:1390522-1390782 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189178 NZ_CP090682:c1507691-1507521 [Burkholderia multivorans]
GTGCTCGACGAAGGGGTCTGCGAACTCAAGATCGACTTTGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGT
GGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCAACGCAACGGAAGGACGTCAAGCGCGCCGTGCAGATGGCGCGCGAAC
TGAAGGAGTAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6