Detailed information of TA system TA189177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1245916..1246484
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JN146_RS05975
• Protein ID: WP_003909358.1
• Coordinates: 1246110..1246484 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN146_RS05970
• Protein ID: WP_003405863.1
• Coordinates: 1245916..1246113 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS05950	1241957..1242595	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN146_RS05955	1242685..1243692	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN146_RS05960	1243709..1244692	-	984	WP_244748916.1	hypothetical protein	-
JN146_RS05965	1244755..1245828	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN146_RS05970	1245916..1246113	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN146_RS05975	1246110..1246484	+	375	WP_003909358.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN146_RS05980	1246687..1247385	+	699	WP_003909359.1	hypothetical protein	-
JN146_RS05985	1247503..1247688	+	186	WP_003901093.1	hypothetical protein	-
JN146_RS05990	1247615..1247890	-	276	WP_003405867.1	hypothetical protein	-
JN146_RS05995	1248133..1248456	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN146_RS06000	1248471..1249169	-	699	WP_031667523.1	hypothetical protein	-
JN146_RS06005	1249203..1249412	+	210	WP_003911400.1	hypothetical protein	-
JN146_RS06010	1249364..1250134	-	771	Protein_1185	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13391.51 Da
• Isoelectric Point: 4.9046

>T189177 WP_003909358.1 NZ_CP069067:1246110-1246484 [Mycobacterium tuberculosis]
VILVDTSVWIEHLCAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189177 NZ_CP090681:2106286-2106636 [Burkholderia multivorans]
ATGGTGAAGCGCGTCAAGTTCGAACGAGGCGATATCGTGCGCGTAAGCCTGAATCCGACTCTCGGTCGTGAGCAGCAGGG
CGACTTTCGTCCGGCGCTGGTGCTCTCCCCGGCAGCGTTCAACGCTTTGGGCGTTGCGCTGGTCGCACCGATCACCCAGG
GTGGCGAATTTGCCCGGTTCGCAGGGTTCGCGGTCCCGCTGTCCGGTGCAGGAACCGCGACGCAGGGCGTCGCGCTGGTG
AACATGGTTCGCATGCTCGACCTGGAGGCGCGCGGCGCTCGCAAGGTCGAACGCGCGCCTGTCGAGGTCGTGGAAGATGC
ACTCGCCCGGCTTCAGACGATCATCGAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189177 WP_003405863.1 NZ_CP069067:1245916-1246113 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189177 NZ_CP090681:2106041-2106292 [Burkholderia multivorans]
ATGGAACTCAAGATTCAAAAATGGGGCAATAGTGCGGCGGTTCGTTTGCCCAGCGTTCTGCTCGAACAGATCAACGCGAC
GGTCGGCAGTTCGCTGAGCGCGGACGTGCGCCCGGATGGCGTGTTGTTGACGCCGGCCCGCCGCAAGTATTCGCTCGATG
ATCTCGTTGCCCAATGCGACACGAAAGCGCCTTTCCCTGCCGACATGGGTGCATGGGGCGAGGTCAAGCCGGTGGGGCGT
GAGGCATGGTGA

Structures

Antitoxin

• AlphaFold DB: G0TH07