Detailed information of TA system TA189174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1026936..1027909
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JN146_RS04890
• Protein ID: WP_003909316.1
• Coordinates: 1027409..1027909 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: JN146_RS04885
• Protein ID: WP_003404754.1
• Coordinates: 1026936..1027412 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS04865	1022783..1024054	-	1272	WP_003909315.1	PPE family protein	-
JN146_RS04870	1024069..1024368	-	300	WP_003404748.1	PE family protein	-
JN146_RS04875	1024812..1026593	+	1782	WP_003404749.1	BCCT family transporter	-
JN146_RS04880	1026593..1026847	+	255	WP_003898646.1	hypothetical protein	-
JN146_RS04885	1026936..1027412	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
JN146_RS04890	1027409..1027909	+	501	WP_003909316.1	GNAT family N-acetyltransferase	Toxin
JN146_RS04900	1028222..1029541	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
JN146_RS04905	1029829..1030410	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
JN146_RS04910	1030410..1032062	+	1653	WP_003404761.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1026590..1079988	53398

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18032.50 Da
• Isoelectric Point: 7.8659

>T189174 WP_003909316.1 NZ_CP069067:1027409-1027909 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPPHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T189174 NZ_CP090681:c1481977-1481081 [Burkholderia multivorans]
ATGAGTGTCGTCGACAAACGAGAACGCGACCGCACCGCGGCGTTCGGCACGCTGCTCGAGCGGTTTCCCGAGCAGCGCTG
CGCCGCCGGTGCGGCAGGCACGATCGGTTATCGCGAGGCCGGTGCGCAGCATGCCGGCGCCGCGCTGGCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCTTCGCGCCGCGTGCTCGCGTGG
GACGCGCCCGGCTACGGCGCATCGACGCCGGTGCACGGCGCATCGCCGTCGGCCGTCGATTACGCGGCGTCGCTCGACGC
CTGGCTCGATGCGCTCGGCATCGCGCGCTGCGTGCTCGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGCATGGTGC
GCGTGAAGCCCGAGCGGATCGCGGGGCTGCTGCTGATTTCGCCGGCCGGCGGTTACGGCAGTGCGCCGGCCGAGACGCGC
GCGTCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGGCCTGCCGGGCTCGCCGCGCAGCGCAGCGGCAACAT
GCTGTCGGGCTTCGCGACCGAAGACGCGCGCGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCCAATGCCGATCTCGCGGCCGATCTCGCCGGGTTTCGCGGCCGCACGGCGGTGGCCGTCGGC
GCGAACGACGCGATTACGCCGCCCGCGGCGTGTGAGCGAATCGCGGCGGCGGCCGGCGTCGGACTGCAGGTGATTCCGCA
GGCCGGCCACGCGGGCTACGTCGAGGCGCCCGACGTCTATTCGGCCTTGATCGACACGTTCTGCCGGCAGTGCGACATCG
AGCGGAGGGCGGCATGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT189174 WP_003404754.1 NZ_CP069067:1026936-1027412 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT189174 NZ_CP090681:c1481084-1480230 [Burkholderia multivorans]
ATGAGCGAACCGTTGCAGAGCGCGGCCGAACCCGCGAAGGCCGAAGCGAGCTACGTCGTGCCGGGCCTCGAGCGCGGGCT
GCGGATTCTCGCCGAATTCTCGCCGCGCGAACCGGTACTCGGTGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACCGTATTCCGTCTGCTGCAGACGCTCGAATCTCTGGGCTTTCTCGAGCGCGCCGACAAGGACCGCAACTACAAGCTC
GGCATCGCGGTGCTGCGGCTCGGCTTCGAATATCTGAGCTCGCTCGAGCTGACCGATCTCGGCCTGCCCGTGATCGAAAG
CCTGCGCGATGCGACCGGCTTCACGACGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCCAAGGCGCAGA
GCGAGGCGCCCGTGTTCAGTTCGATTCGCGTGAACGTCGGTACGCGGCTGCCCGCGCACGCGACGACGCACGGTCACGTG
CTGATGGGCGACCTCTCGCTGGACGAGCTGCGCGCGCTGTATCCGGAAGGCACGCTGCCGCGGATGACGAACGCGACGCC
GGACACGGTCGATGCGCTGTACACCATGATCCGCGAGGATGCGCTGCGCGGATACGGCATCAGCAATTCGTCGTTCGAGC
GCGGGATTTCGGTCGTCACCGCGCCGGTACGCAACGACACGCAGAAGATCGTCGCCTGCATCACGGTGACGGTGCCGCGC
CCGGAGATCGACGCGACGCTGATCGCCGATGGGCTGATCGACAAGGTGCGGCGTGCGGCGGCCGAACTGTCGCGGCGGCT
CAATTACCGCTCGGACGACGAGCACACCTTTTTGAAGGCTTTGGGACTCCGATGA

Structures

Antitoxin

• AlphaFold DB: G0TFR4