Detailed information of TA system TA189173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1017406..1018025
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN146_RS04835
• Protein ID: WP_003404726.1
• Coordinates: 1017591..1018025 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: JN146_RS04830
• Protein ID: WP_003404724.1
• Coordinates: 1017406..1017585 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS04815	1012879..1014459	+	1581	WP_003909313.1	serine hydrolase	-
JN146_RS04820	1014456..1016849	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JN146_RS04825	1017122..1017280	+	159	WP_003404720.1	hypothetical protein	-
JN146_RS04830	1017406..1017585	+	180	WP_003404724.1	antitoxin	Antitoxin
JN146_RS04835	1017591..1018025	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN146_RS04840	1018123..1018896	+	774	WP_003404735.1	VOC family protein	-
JN146_RS04845	1018961..1019410	+	450	WP_003404738.1	hypothetical protein	-
JN146_RS04850	1019545..1019775	-	231	WP_003898642.1	hypothetical protein	-
JN146_RS04855	1019942..1021450	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
JN146_RS04860	1021452..1022690	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T189173 WP_003404726.1 NZ_CP069067:1017591-1018025 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T189173 NZ_CP090681:1213802-1214098 [Burkholderia multivorans]
ATGGAAAAGAGAACGCCGCATTGCAAGCTGCCGCGCGTCAAGGCGCTGATCGAAGCCGGCAAGGTCCGCCTGACCGCAAG
CGCCGTCATCGGCGCCCGCCAGATGGGCTTCACCGAAGCGGAAACGATCGACGTGGTAAGGGCGCTGACACCGTTCGAAT
TCCACAAGAGCATGACGACCTACGCCGACCACACGATCTGGCAGGACGTCTACCGGCCTTGCACGATGCGCGGCGACGTG
TATCTGAAACTGACCGTCATCGACGATGTGCTGATCGTGTCTTTCAAGGAGCGATGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT189173 WP_003404724.1 NZ_CP069067:1017406-1017585 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT189173 NZ_CP090681:1214100-1214501 [Burkholderia multivorans]
ATGAAATGTCCTGCATGCGGTGCTGCCGCGCTGGTCCGCGACACGCGCGACATCCCGTACACCTACAAGGGCGAATCGAC
CGTGATCCCGCACGTCACGGGCGAGTTTTGCCCCGCGTGCGGCGAGTCGGTGCTCGACGTCGGCGAGGCGACCCGTTTCG
GCGAAGCAGTGTCGGCGTTCAACCGGCAAGTGAACGCGACACTCGTTGATCCGAAGTTCATTGCGACGGTGCGCAAGAAG
CTGAAGCTCGATCAGCGCGAGGCGGCCGAGATTTTCGGCGGCGGCGTCAACGCGTTTTCCCGCTACGAGACCGGCAAGAC
CAATCCGCCGCTGGCACTCGTGAAGCTGCTGAAGCTTCTCGACCGTCATCCCGATCTGCTCGACGAAGTGCGTGCGGCAT
AA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5