Detailed information of TA system TA189163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 700612..701258
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN146_RS03165
• Protein ID: WP_003403137.1
• Coordinates: 700612..701025 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN146_RS03170
• Protein ID: WP_003403139.1
• Coordinates: 701022..701258 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS03145	696695..698245	+	1551	WP_244749062.1	MlaD family protein	-
JN146_RS03150	698297..698689	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN146_RS03155	698686..698943	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN146_RS03160	699126..700361	-	1236	WP_003403128.1	ATP-binding protein	-
JN146_RS03165	700612..701025	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN146_RS03170	701022..701258	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN146_RS03175	701362..701868	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN146_RS03180	701982..702512	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN146_RS03185	702496..703191	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN146_RS03190	703314..703625	+	312	WP_003403164.1	hypothetical protein	-
JN146_RS03195	703697..704650	+	954	WP_031667544.1	DUF1259 domain-containing protein	-
JN146_RS03200	704891..705475	+	585	WP_031667543.1	IS607-like element IS1536 family transposase	-
JN146_RS03205	705477..706188	+	712	Protein_636	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T189163 WP_003403137.1 NZ_CP069067:c701025-700612 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T189163 NZ_CP090678:c1569592-1568696 [Burkholderia multivorans]
ATGAGTGTCGTCGACAAACGAGAACGCGACCGCACCGCGGCGTTCGCCACGCTGCTCGAGCGGTTTCCCGAGCAGCGCTG
CGCCGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGTGCGCAGCAGGCTGGCGCCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCTTCGCGCCGCGTGCTCGCGTGG
GACGCGCCCGGCTACGGCGCATCGACGCCGGTGCACGGCGCATCGCCGTCGGCCGTCGATTACGCGGCGTCGCTCGACGC
GTGGCTCGACGCGCTCGGCATCGCGCGCTGCGTGCTCGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGCATGGTGC
GCGTGAAGCCCGAGCGGATCGCGGGGCTGCTGCTGATTTCGCCGGCCGGCGGATACGGCAGCGCGCCGGCCGAGACGCGC
GCGTCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGGCCTGCCGGGCTCGCCGCGCAGCGCAGCGGCAACAT
GCTGTCGGGCTTCGCGACCGAGGACGCGCGCGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCCAATGCCGATCTCGCGGCCGATCTCGCCGGGTTCCGCGGCCGCACGGCGGTGGCCGTGGGC
GCGAACGACGCGATTACGCCGCCCGCGGCGTGCGAACGCATCGCGGCGGCCGCCGGCGTCGGACTGCAGGTGATTCCGCA
GGCCGGCCACGCGGGCTACGTCGAGGCGCCCGACGTCTATTCGGCCTTGATCGACACGTTCTGCCGGCAGTGCGACATCG
AGCGGAGGGCGGCATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT189163 WP_003403139.1 NZ_CP069067:c701258-701022 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT189163 NZ_CP090678:c1568699-1567845 [Burkholderia multivorans]
ATGAGCGAACCGTTGCAGAGCGCGGCCGAACCTGCGAAGGCCGAAGCGAGCTACGTCGTGCCCGGCCTCGAGCGCGGGCT
GCGGATTCTCGCCGAATTCTCGCCGCGCGAACCGGTACTCGGCGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACCGTATTCCGTCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCCGACAAGGACCGCAACTACAAGCTC
GGCATCGCGGTGCTGCGGCTCGGCTTCGAATATCTGAGCTCGCTCGAGCTGACCGATCTCGGCCTGCCCGTGATCGAAAG
CCTGCGCGATGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTCGTGTTCGTTGCGAAGGCGCAGA
GCGAGGCGCCCGTGTTCAGTTCGATTCGCGTGAACGTCGGTACGCGGCTGCCCGCGCACGCGACGACGCACGGTCACGTG
CTGATGGGCGACCTGTCGCTGGACGAGCTGCGCGCGCTGTATCCGGAAGGGACGCTGCCGCGGATGACGAACGCGACGCC
CGACACGGTCGACGCGCTGTACACCATGATCCGCGAGGATGCGCTGCGCGGATACGGCATCAGCAATTCGTCGTTCGAGC
GCGGGATTTCGGTCGTCACCGCGCCGGTGCGCAACGACACGCAGAAGATCGTCGCCTGCATCACGGTGACGGTGCCGCGC
CCGGAGATCGACGCGACGCTGATCGCCGACGGGCTGATCGACAAGGTGCGGCGCGCGGCGGCTGAACTGTCGCGGCGGCT
CAATTACCGCTCGGACGACGAACACACGTTTTTGAAGGCTTTGGGACTCCGATGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89