Detailed information of TA system TA189152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3751536..3752065
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN147_RS17565
• Protein ID: WP_003417760.1
• Coordinates: 3751808..3752065 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN147_RS17560
• Protein ID: WP_003417757.1
• Coordinates: 3751536..3751811 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS17525	3748109..3748798	-	690	WP_234787042.1	BBE domain-containing protein	-
JN147_RS17530	3748985..3749356	-	372	WP_003417739.1	FAD-binding protein	-
JN147_RS17535	3749254..3749472	-	219	WP_157132559.1	hypothetical protein	-
JN147_RS17540	3749499..3749759	-	261	WP_003417741.1	hypothetical protein	-
JN147_RS17545	3749874..3750263	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN147_RS17550	3750277..3750570	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN147_RS17555	3750567..3751412	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN147_RS17560	3751536..3751811	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN147_RS17565	3751808..3752065	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN147_RS17570	3752107..3753297	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN147_RS17575	3753414..3753782	+	369	WP_003417765.1	FHA domain-containing protein	-
JN147_RS17580	3753779..3754330	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN147_RS17585	3754337..3754918	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN147_RS17590	3754899..3755267	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN147_RS17595	3755245..3755637	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189152 WP_003417760.1 NZ_CP069066:3751808-3752065 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189152 NZ_CP090676:c734648-734325 [Burkholderia cenocepacia]
ATGAATCGCGGGAGCATTGTCACAGTCGCGCTCCAAGGTGACTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGCGTAACGTTGCTGCTAATGTCGAGCGCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189152 WP_003417757.1 NZ_CP069066:3751536-3751811 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189152 NZ_CP090676:c734857-734645 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCCGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGTCCAATTCAGATATG
GGTGCCGGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGTTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94