Detailed information of TA system TA189151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3687875..3688549
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: JN147_RS17360
• Protein ID: WP_003417282.1
• Coordinates: 3687875..3688303 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: JN147_RS17365
• Protein ID: WP_003417286.1
• Coordinates: 3688307..3688549 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS17335	3683697..3684098	-	402	WP_003417264.1	cytidine deaminase	-
JN147_RS17340	3684335..3684673	+	339	WP_003417267.1	succinate dehydrogenase, cytochrome b556 subunit	-
JN147_RS17345	3684670..3685104	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
JN147_RS17350	3685233..3687005	+	1773	WP_031652407.1	succinate dehydrogenase flavoprotein subunit	-
JN147_RS17355	3687005..3687796	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
JN147_RS17360	3687875..3688303	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN147_RS17365	3688307..3688549	-	243	WP_003417286.1	CopG family transcriptional regulator	Antitoxin
JN147_RS17370	3688671..3689285	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
JN147_RS17375	3689282..3689860	-	579	Protein_3435	molybdenum cofactor biosynthesis protein MoaE	-
JN147_RS17385	3691213..3691305	-	93	Protein_3437	molybdopterin converting factor subunit 1	-
JN147_RS17390	3691306..3691839	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
JN147_RS17395	3691836..3692210	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
JN147_RS17400	3692307..3693311	-	1005	WP_234787058.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T189151 WP_003417282.1 NZ_CP069066:c3688303-3687875 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T189151 NZ_CP090676:c298996-298595 [Burkholderia cenocepacia]
ATGCCGCGCTTCATGCTCGACACCAATATGTGTATCTATCTGATGAAAAATCAGCCTGAGCAGGTCGCGAGACGGTTTGC
GCGGTGCTACACGGGCGACGTCGTCATGTCGGCGATCACCTATGCGGAGCTCGAGTGCGGCCTGACCGCTTGTGTGAATC
CCGCGCGGGAGCGCCGCCATCTCGCCGCATTGATCGAGGATATTCCCGTCGCGCCTTTCGACGCGGCTGCCGCGCAAGCG
TATGGCCCGGTTCGCGAGGCCACCCGCGAGCGGAAGAAGGATCACCTCGACAAACTGATTGCCGCGCATGCGGTGTCGCT
CGATGTCGTCCTTGTCACCAACAACGAGCGCGATTTCGTCAGCTATCCGGGATTGCGACTGGAGAACTGGCTGAACGACT
AG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT189151 WP_003417286.1 NZ_CP069066:c3688549-3688307 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT189151 NZ_CP090676:c299232-298996 [Burkholderia cenocepacia]
ATGCATACCACGAGGGTATTCAGGAACGGCAACTCGCAGGCCGTGCGAATTCCTGCGGACCTTGCCTATGAGCGCAGCGA
TATCGAACTGGAAATTGAGCGCGTCGGTGACGAAATCCGCATTCGGCCGATGCGGCGGCCTTTGACTCAGGTACTGGCGA
AGTTCGCGAAATTCGGGCCGGACTTCATGGCCGATGGGCGTGGCGACCACGAGCAGGCCGAACGCGAGGGTTTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59