Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3162150..3162837 Replicon chromosome
Accession NZ_CP069066
Organism Mycobacterium tuberculosis strain N1201

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag JN147_RS14990 Protein ID WP_003414624.1
Coordinates 3162394..3162837 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag JN147_RS14985 Protein ID WP_003414620.1
Coordinates 3162150..3162407 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN147_RS14965 3157470..3158324 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
JN147_RS14970 3158380..3159543 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
JN147_RS14975 3159560..3160774 - 1215 WP_003414610.1 zinc metalloprotease Rip -
JN147_RS14980 3160782..3162023 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
JN147_RS14985 3162150..3162407 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
JN147_RS14990 3162394..3162837 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
JN147_RS14995 3162917..3163579 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
JN147_RS15000 3163675..3163866 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
JN147_RS15005 3164198..3165946 + 1749 WP_201030602.1 cytochrome c biogenesis protein DipZ -
JN147_RS15010 3166042..3166623 + 582 WP_003414644.1 fasciclin domain-containing protein -
JN147_RS15015 3166723..3166989 + 267 WP_031652375.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T189149 WP_003414624.1 NZ_CP069066:3162394-3162837 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T189149 NZ_CP090673:2107547-2107924 [Burkholderia multivorans]
ATGGGTATAAATTCATCGATGGTTCCGTACACCAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGCACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGTCTCGGATCGGCCGGCGTACTCGAGGTCGTGGAGTCGGAGGACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCTACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGGGCAGCCGAAGCACACGCAAATGGAGAAAAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT189149 WP_003414620.1 NZ_CP069066:3162150-3162407 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT189149 NZ_CP090673:2107924-2108247 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTTGGCACGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAGATCATCAAGACTCGCAAATGGACGCAGCAGCACGCAGCACGATTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGTGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References