Detailed information of TA system TA189147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3115634..3116238
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: JN147_RS14765
• Protein ID: WP_003414492.1
• Coordinates: 3115634..3116026 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN147_RS14770
• Protein ID: WP_003910640.1
• Coordinates: 3116023..3116238 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS14735	3110784..3111572	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
JN147_RS14740	3111906..3112451	-	546	WP_031652371.1	DUF1802 family protein	-
JN147_RS14745	3112723..3113607	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JN147_RS14750	3113610..3114497	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
JN147_RS14755	3114802..3115347	-	546	WP_003899500.1	DUF1802 family protein	-
JN147_RS14760	3115344..3115613	-	270	WP_003414489.1	DUF2277 family protein	-
JN147_RS14765	3115634..3116026	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN147_RS14770	3116023..3116238	-	216	WP_003910640.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
JN147_RS14775	3116285..3117034	+	750	WP_003414497.1	enoyl-CoA hydratase	-
JN147_RS14780	3117113..3118195	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
JN147_RS14785	3118188..3119498	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
JN147_RS14790	3119501..3120328	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
JN147_RS14795	3120325..3121236	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T189147 WP_003414492.1 NZ_CP069066:c3116026-3115634 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T189147 NZ_CP090673:c1074159-1073875 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGACGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7495.55 Da
• Isoelectric Point: 4.9732

>AT189147 WP_003910640.1 NZ_CP069066:c3116238-3116023 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVAWNVS

Nucleotide

• Length: 216 bp

>AT189147 NZ_CP090673:c1074456-1074163 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAACTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCCCGTGCAGATGGCGCGCGAACTGAAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8