Detailed information of TA system TA189143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3049571..3050250
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN147_RS14395
• Protein ID: WP_003414059.1
• Coordinates: 3049571..3049987 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN147_RS14400
• Protein ID: WP_003414061.1
• Coordinates: 3049984..3050250 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS14370	3045623..3046525	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN147_RS14375	3046594..3047346	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN147_RS14385	3047590..3047865	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN147_RS14390	3047862..3049484	-	1623	WP_031652369.1	class I SAM-dependent DNA methyltransferase	-
JN147_RS14395	3049571..3049987	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN147_RS14400	3049984..3050250	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN147_RS14405	3050276..3050671	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN147_RS14410	3050668..3050937	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN147_RS14415	3050947..3052041	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN147_RS14420	3052038..3052457	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN147_RS14425	3052456..3052530	+	75	Protein_2848	hypothetical protein	-
JN147_RS14430	3052531..3053010	-	480	WP_003414073.1	dihydrofolate reductase	-
JN147_RS14435	3053081..3053881	-	801	WP_003911953.1	thymidylate synthase	-
JN147_RS14440	3054037..3054774	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189143 WP_003414059.1 NZ_CP069066:c3049987-3049571 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189143 NZ_CP090672:c1227522-1227223 [Burkholderia multivorans]
ATGCCGGTTAAGGCGCGCACGGTACGCCTGACGCCGCTGGCGGAGGCCGACCTCGAGGCCATCTGGTCTTACACGTACAA
GCGCTGGTCGCTCGACCAGGCTGAGCGCTATATCGGCGAGCTGAGCGCGGCGTTCGAGCGGCTGGCGCGCCGGGAGTCGG
TAGGGTGGCCGAGCCGCGCGGGCGGCAACTATTCGCGGTACCTGGTCGGTTCGCACGTGGTGTTCTATCGGGAAACGACC
GAAACCCTTGACGTCATTCGCGTGCTGCACCAGCGGATGGATGTCGACCGACATCTGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189143 WP_003414061.1 NZ_CP069066:c3050250-3049984 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189143 NZ_CP090672:c1227757-1227512 [Burkholderia multivorans]
ATGGCACGAAATACATCGATCTCGCTGGGCGATCATCTGACCGAATTTGTCGACATGCAGGTCGCGTCCGGCCGCTACGG
CTCGGCTAGCGATGTCGTGCGCGCGGGCCTGCGCCTGCTCGAGACACAGGAAACCGAGCTGCGGGCGCTGCAGGAAGCGT
TGAAGGCGGGCGAGGCATCGGGCACACCGGCTCCGTTCGACAGCCAGGCGTTCCTGGCCCGGATGCGGGCCAAGCATGCC
GGTTAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64