Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2860016..2860653 Replicon chromosome
Accession NZ_CP069066
Organism Mycobacterium tuberculosis strain N1201

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN147_RS13375 Protein ID WP_003413180.1
Coordinates 2860016..2860411 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN147_RS13380 Protein ID WP_003413183.1
Coordinates 2860408..2860653 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN147_RS13335 2856101..2856760 + 660 WP_003900846.1 LppA family lipoprotein -
JN147_RS13340 2856757..2857416 + 660 WP_003900847.1 LppA family lipoprotein -
JN147_RS13345 2857413..2858075 + 663 WP_003900848.1 LppA family lipoprotein -
JN147_RS13350 2858072..2858350 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN147_RS13355 2858443..2858856 + 414 WP_003413164.1 PIN domain nuclease -
JN147_RS13360 2858895..2859152 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN147_RS13365 2859149..2859526 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN147_RS13370 2859542..2859916 - 375 WP_003413177.1 hypothetical protein -
JN147_RS13375 2860016..2860411 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN147_RS13380 2860408..2860653 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN147_RS13385 2861064..2861483 - 420 WP_003413190.1 A24 family peptidase -
JN147_RS13390 2861495..2862304 - 810 WP_003910591.1 shikimate dehydrogenase -
JN147_RS13395 2862301..2863554 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN147_RS13400 2863547..2864059 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189141 WP_003413180.1 NZ_CP069066:c2860411-2860016 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189141 NZ_CP090672:792071-792490 [Burkholderia multivorans]
ATGATCCTTGTCGATACGAACGTCATTTTCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
CCAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGAC
GCCGGCGGGACTGGCTGGAGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGGGCAAAGCCTATGCGCGCATTCGTGCGCACGCCCGTGCCGTCGGCAACGCGATCGCGCCGGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189141 WP_003413183.1 NZ_CP069066:c2860653-2860408 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189141 NZ_CP090672:791901-792074 [Burkholderia multivorans]
CTGCACGGGCGCAGTACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTGGG
AACCTTGCTGGCGGAAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATCCAGCGTGACAAGACGCGAACCGACCCGA
TGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References