Detailed information of TA system TA189138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2843900..2844540
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN147_RS13265
• Protein ID: WP_003412970.1
• Coordinates: 2843900..2844319 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN147_RS13270
• Protein ID: WP_003910588.1
• Coordinates: 2844316..2844540 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS13235	2839488..2840210	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN147_RS13240	2840727..2840954	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN147_RS13245	2840951..2841352	+	402	WP_003412963.1	PIN domain-containing protein	-
JN147_RS13250	2841387..2842307	-	921	WP_003412965.1	restriction endonuclease	-
JN147_RS13255	2842648..2842854	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN147_RS13260	2842952..2843899	+	948	WP_162097749.1	ERCC4 domain-containing protein	-
JN147_RS13265	2843900..2844319	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN147_RS13270	2844316..2844540	-	225	WP_003910588.1	hypothetical protein	Antitoxin
JN147_RS13275	2844571..2847414	-	2844	WP_003910589.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN147_RS13280	2847486..2847887	-	402	WP_003412981.1	hypothetical protein	-
JN147_RS13285	2847887..2848357	-	471	WP_003412985.1	transcription antitermination factor NusB	-
JN147_RS13290	2848360..2848923	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189138 WP_003412970.1 NZ_CP069066:c2844319-2843900 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189138 NZ_CP090650:c171637-171335 [Pseudomonas aeruginosa]
ATGAAAAAGATCGAATCCAGCAAGTTCCGAACTTGGCTGAAGAAGCTCAAGGACGACACCGGCAGGGCCCGTATCGTGGC
GCGGATCAATCGGCTGATGGAGGGCTTGCCGGGCGATGTCGCTCCGGTCGGGCACGGCGTCAGCGAGCTACGGATTCACT
ACGGCCCCGGCTACCGGGTGTATTTTCACCAGCAAGGCGACACCCTGGTGATACTGCTCTGCGGTGGCGACAAGGGCAGC
CAGAGCCGTGACATCGAGGCCGCCCACCAGATTCTGCAGGCGTGGAGAGCGCAACATGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8021.99 Da
• Isoelectric Point: 4.0057

>AT189138 WP_003910588.1 NZ_CP069066:c2844540-2844316 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDVRSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189138 NZ_CP090650:c171342-171049 [Pseudomonas aeruginosa]
ATGACTGAGACCTTTACCGATTTCGACCCCGCCGAGTACCTCACCAGCCCCGAGGCCATTGCGGAGTTTATGAGCGATGC
CCTGGAAACCGGCGATGCCGGTTATATCGCCAAGGCCATGGGGGTGGTGGCGCGCGCCAGAGGCATGACCGAGCTGGCCA
GGGAAACCGGCCTGTCCCGCGAGCAGCTGTACCGTTCCTTCAGCGAGCACGGCAATCCCACGCTGAAAACCATGCTCGCC
GTCATGAAGGCGCTGGGCGTCGATATGGCTGCTCGCCCGCATATCGCGCACTGA

Structures

Toxin

• AlphaFold DB: G0TQ08