Detailed information of TA system TA189123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1770664..1771277
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: JN147_RS08305
• Protein ID: WP_003407786.1
• Coordinates: 1770873..1771277 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: JN147_RS08300
• Protein ID: WP_009935474.1
• Coordinates: 1770664..1770867 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS08285	1765928..1768831	+	2904	WP_003904644.1	MMPL family transporter	-
JN147_RS08290	1768841..1769287	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
JN147_RS08295	1769322..1770611	+	1290	WP_003407781.1	threonine ammonia-lyase	-
JN147_RS08300	1770664..1770867	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN147_RS08305	1770873..1771277	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
JN147_RS08310	1771294..1773036	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
JN147_RS08315	1773029..1775326	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T189123 WP_003407786.1 NZ_CP069066:1770873-1771277 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T189123 NZ_CP090648:c1520504-1520226 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT189123 WP_009935474.1 NZ_CP069066:1770664-1770867 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT189123 NZ_CP090648:c1520214-1519909 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46