Detailed information of TA system TA189118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1382992..1383680
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: JN147_RS06610
• Protein ID: WP_003406304.1
• Coordinates: 1383249..1383680 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: JN147_RS06605
• Protein ID: WP_003406302.1
• Coordinates: 1382992..1383252 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS06585	1378569..1379393	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
JN147_RS06590	1379398..1380579	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
JN147_RS06595	1380656..1381756	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
JN147_RS06600	1381927..1382916	+	990	WP_003910306.1	malate dehydrogenase	-
JN147_RS06605	1382992..1383252	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
JN147_RS06610	1383249..1383680	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN147_RS06615	1383703..1385390	-	1688	Protein_1307	PE family protein	-
JN147_RS06620	1385570..1386430	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
JN147_RS06625	1386511..1387341	-	831	WP_003910309.1	SDR family oxidoreductase	-
JN147_RS06630	1387398..1387691	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
JN147_RS06635	1387688..1387957	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189118 WP_003406304.1 NZ_CP069066:1383249-1383680 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189118 NZ_CP090647:731608-731886 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189118 WP_003406302.1 NZ_CP069066:1382992-1383252 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189118 NZ_CP090647:731898-732203 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6