Detailed information of TA system TA189117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1237342..1237910
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN147_RS05945
• Protein ID: WP_003405865.1
• Coordinates: 1237536..1237910 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN147_RS05940
• Protein ID: WP_003405863.1
• Coordinates: 1237342..1237539 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS05920	1233383..1234021	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN147_RS05925	1234111..1235118	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN147_RS05930	1235135..1236118	-	984	WP_003898731.1	hypothetical protein	-
JN147_RS05935	1236181..1237254	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
JN147_RS05940	1237342..1237539	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN147_RS05945	1237536..1237910	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN147_RS05950	1238113..1238811	+	699	WP_003898733.1	hypothetical protein	-
JN147_RS05955	1238929..1239114	+	186	WP_003901093.1	hypothetical protein	-
JN147_RS05960	1239041..1239316	-	276	WP_003405867.1	hypothetical protein	-
JN147_RS05965	1239559..1239882	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN147_RS05970	1239897..1240603	-	707	Protein_1177	guanylate cyclase	-
JN147_RS05975	1240625..1240834	+	210	WP_244747409.1	hypothetical protein	-
JN147_RS05980	1240786..1241556	-	771	Protein_1179	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189117 WP_003405865.1 NZ_CP069066:1237536-1237910 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189117 NZ_CP090647:c142355-142074 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATTGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189117 WP_003405863.1 NZ_CP069066:1237342-1237539 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189117 NZ_CP090647:c142579-142352 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07