Detailed information of TA system TA189114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1021326..1022299
• Replicon: chromosome
• Accession: NZ_CP069066
• Organism: Mycobacterium tuberculosis strain N1201

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JN147_RS04870
• Protein ID: WP_031652172.1
• Coordinates: 1021799..1022299 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: JN147_RS04865
• Protein ID: WP_003404754.1
• Coordinates: 1021326..1021802 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN147_RS04845	1017173..1018444	-	1272	WP_003404747.1	PPE family protein	-
JN147_RS04850	1018459..1018758	-	300	WP_003404748.1	PE family protein	-
JN147_RS04855	1019202..1020983	+	1782	WP_003404749.1	BCCT family transporter	-
JN147_RS04860	1020983..1021237	+	255	WP_003898646.1	hypothetical protein	-
JN147_RS04865	1021326..1021802	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
JN147_RS04870	1021799..1022299	+	501	WP_031652172.1	GNAT family N-acetyltransferase	Toxin
JN147_RS04880	1022612..1023931	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
JN147_RS04885	1024219..1024800	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
JN147_RS04890	1024800..1026452	+	1653	WP_003910231.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18094.63 Da
• Isoelectric Point: 7.7931

>T189114 WP_031652172.1 NZ_CP069066:1021799-1022299 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRCLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T189114 NZ_CP090645:c80531-80100 [Burkholderia contaminans]
ATGACCTACCTGCTCGACGTCAACGTCCTTATCGCCTTGCTGGATCCTGCTCATGTTCAGCACGAACCGGCGCATGCATG
GTTTGGCCGGGTAGGGTATGCCGCATGGTCGACTTGCCCTCTGACTGAGAACGGCGTGTTGCGGATTATCGGCAATCCGA
AGTATCCGAACACGATGGGGTCGCCGGCCTCCGTCGCCCCGCTCGTCGCGCAACTGCGGGCGCACGCCGGGCACACGTTC
TGGCCGGATGATGTGAGCTTGCTGGACGAGAAGCACGTCGACGCCACGCGCTTACTGTCGTCGGCTCAGTTGACCGATAC
GTACTTGCTGGCGCTGGCGCGCGTACACAAGGGCAAGCTGGCGACCTTCGATCGGCGCCTGGTTGTCGACGCCGTGCCGA
ATGGCGCCAAGCATCTCGAACTGATTTCCTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT189114 WP_003404754.1 NZ_CP069066:1021326-1021802 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT189114 NZ_CP090645:c80758-80531 [Burkholderia contaminans]
ATGCGTACCACTATCGACATCGATGACGACATCCTCGCCCTCGCACGGGCGCGTGCGAAGCCCGGCACGAGTATCGGCAA
GGTCATTTCCGAGCTCGCGCGCGCCGCATTGCAACGCACGACGACCAACCAGACGAGCCGTAACGGACTGCCGTTGATGC
CGACCTCCGCGACGGCCCGGTCGGTCACGCTCGACACCGTCAATCAACTTCGCGACGATTCACTTTGA

Structures

Antitoxin

• AlphaFold DB: G0TFR4