Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 753300..754092 Replicon chromosome
Accession NZ_CP069066
Organism Mycobacterium tuberculosis strain N1201

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag JN147_RS03495 Protein ID WP_003403386.1
Coordinates 753655..754092 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag JN147_RS03490 Protein ID WP_003403381.1
Coordinates 753300..753545 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN147_RS03460 748320..749825 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
JN147_RS03465 749906..750916 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
JN147_RS03470 751304..751687 - 384 WP_003403365.1 ribonuclease VapC6 -
JN147_RS03475 751782..751937 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
JN147_RS03480 752013..752729 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
JN147_RS03485 753005..753313 - 309 WP_031652154.1 type II toxin-antitoxin system PemK/MazF family toxin -
JN147_RS03490 753300..753545 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
JN147_RS03495 753655..754092 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
JN147_RS03500 754089..754343 - 255 WP_003911263.1 antitoxin VapB7 -
JN147_RS03505 754457..756825 + 2369 Protein_693 arylsulfatase AtsD -
JN147_RS03510 756888..757214 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN147_RS03515 757126..757464 + 339 WP_003403405.1 PIN domain-containing protein -
JN147_RS03520 757461..757634 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T189111 WP_003403386.1 NZ_CP069066:c754092-753655 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T189111 NZ_CP090644:65594-65887 [Burkholderia contaminans]
ATGAGAATCGAGCAGACAGACGACTTCGCGAAATGGTTGCGCGGCCTACGCGATCACATCGCGCGCGCGCAGATCGCCAA
GCGGATTCAACGGCTCGCGCGCGGCCAATATGGGGACATGAAGCCGGTGGGTGCCGGAGTGAGCGAAATGCGCGTGCACG
TCGGCCCCGGCTATCGCGTCTACTTTGTCCAACGAGGCTCAACGCTCGTCGTCCTGCTATGCGGTGGCGACAAGTCAACG
CAGCAGCGCGACATTGAGCGTGCTATCGCGCTTTCGGCGCAATTGGGAGATTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189111 WP_003403381.1 NZ_CP069066:c753545-753300 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189111 NZ_CP090644:65889-66188 [Burkholderia contaminans]
ATGGGTATCAAGACAACGCCGTTCGATGCGGCCGAATACCTCGACACGCCCGAGGCGCAGGCCGAATATCTGCGGATTTC
GTTCGAGACCGGCGATTCCGCCGACATTCAGGAAGCCATCGGCACCGTGGCGCGTGCGCGTGGCATGACGCAGATTGCGC
GCGACGCGGGCGTCGGCCGCGAAAGCCTCTACAAAGCGCTGGACGAGCACGGCAACCCGGAATTCGCGACGATCCTGCGT
GTTGTGCGCGCGCTCGGCCTCACGTTGACCGTGACGACGCCCGTACACGAGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References