Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/Txe-YefM
Location 3751487..3752016 Replicon chromosome
Accession NZ_CP069065
Organism Mycobacterium tuberculosis strain N0091

Toxin (Protein)


Gene name relE Uniprot ID G0TI95
Locus tag JN148_RS17605 Protein ID WP_003417760.1
Coordinates 3751759..3752016 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G0TI94
Locus tag JN148_RS17600 Protein ID WP_003417757.1
Coordinates 3751487..3751762 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN148_RS17565 3748060..3748749 - 690 WP_234787042.1 BBE domain-containing protein -
JN148_RS17570 3748936..3749307 - 372 WP_003417739.1 FAD-binding protein -
JN148_RS17575 3749205..3749423 - 219 WP_157132559.1 hypothetical protein -
JN148_RS17580 3749450..3749710 - 261 WP_003417741.1 hypothetical protein -
JN148_RS17585 3749825..3750214 + 390 WP_003417745.1 DUF732 domain-containing protein -
JN148_RS17590 3750228..3750521 - 294 WP_003417749.1 DUF3017 domain-containing protein -
JN148_RS17595 3750518..3751363 - 846 WP_003417751.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
JN148_RS17600 3751487..3751762 + 276 WP_003417757.1 type II toxin-antitoxin system antitoxin RelJ Antitoxin
JN148_RS17605 3751759..3752016 + 258 WP_003417760.1 Txe/YoeB family addiction module toxin Toxin
JN148_RS17610 3752058..3753248 + 1191 WP_244693047.1 NADH:flavin oxidoreductase -
JN148_RS17615 3753365..3753733 + 369 WP_003417765.1 FHA domain-containing protein -
JN148_RS17620 3753730..3754281 - 552 WP_244693050.1 pentapeptide repeat protein MfpA -
JN148_RS17625 3754288..3754869 - 582 WP_003417769.1 ATP/GTP-binding protein -
JN148_RS17630 3754850..3755218 - 369 WP_003417772.1 DUF742 domain-containing protein -
JN148_RS17635 3755196..3755588 - 393 WP_003417776.1 serine protease inhibitor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(6-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10070.30 Da        Isoelectric Point: 6.4693

>T189094 WP_003417760.1 NZ_CP069065:3751759-3752016 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Download         Length: 258 bp

>T189094 NZ_CP090636:c2758328-2758086 [Burkholderia contaminans]
GTGGCACGTGTCGAGGCACTTCAGTTCGAGGTGGTGTCGTTCGATGTGGAAGATGCCCGGCACGCCGGCGCGATTCGTGC
GCACCCGACTGCGGTCGGCACTCCGATCGGTCCGTACGATGCGTTGATCGCGGGGCAGGCGCGCGCACGGCCTCTGGTGC
TTGTCACGCATAACGTGCGCGAGTTTGCGCGAGTCCCGGGGCTACAGGTCGAGGATTGGCTCGCCAAGGCGCGTGAAGAC
TGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10194.35 Da        Isoelectric Point: 4.6652

>AT189094 WP_003417757.1 NZ_CP069065:3751487-3751762 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Download         Length: 276 bp

>AT189094 NZ_CP090636:c2758701-2758459 [Burkholderia contaminans]
ATGGATACTGCCAGGATTTTCGAGCATGGCGGATCGCAGGCCGTCCTTCTGCCGAAGGAATTCCGCTTCGAAACGACCGA
GGTACGGATTCGTCGCCACGGGTCATCCGTCATCCTCGAACCGGTGCCGCAAGGCGGGGCCTGGCTCACGCCACTGATCG
GCCCGGTCGACGCCGATTTCGAGGCTGCGACTACGACCGGGCCTGCCGCTCAGCAGCGCTCAGGTCTGGACGTATTTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3OEI

Antitoxin

Source ID Structure
PDB 3CTO
PDB 3OEI
PDB 3D55
AlphaFold DB G0TI94

References